NHWD-870

Name: NHWD-870
Brand: TargetMol
SKU: T36573
Rating: 4.5 (1 reviews)

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Catalog No. T36573Cas No. 2115742-03-3

NHWD-870 is an effective and selective inhibitor of BET family bromodomain only binding to BRD2, BRD3, BRD4 (IC50 = 2.7 nM), and BRDT. NHWD-870 exhibits potent anti-tumor efficacies and suppresses cancer cell-macrophage interaction through the increase of tumor apoptosis and inhibition of tumor proliferation.

NHWD-870

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Catalog No. T36573Cas No. 2115742-03-3

Pack Size	Price	Availability
25 mg	$1,630	8-10 weeks
50 mg	$2,120	8-10 weeks
100 mg	$3,100	8-10 weeks

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Product Introduction

Bioactivity

Description	NHWD-870 is an effective and selective inhibitor of BET family bromodomain only binding to BRD2, BRD3, BRD4 (IC50 = 2.7 nM), and BRDT. NHWD-870 exhibits potent anti-tumor efficacies and suppresses cancer cell-macrophage interaction through the increase of tumor apoptosis and inhibition of tumor proliferation.
Targets&IC50	BRD4:2.7 nM
In vitro	NHWD-870 exhibits mild inhibition of the hERG channel (IC50 = 5.4 µM). NHWD-870 shows robust activities inducing apoptosis and suppressing cell proliferation. NHWD-870 (0.01-10000 nM) inhibits melanoma cells (A375) with an IC50 of 2.46 nM. In H526, A2780, ES-2, and MDA-MB231 cells, NHWD-870 (0-10000 nM; 5 days) suppressed cell growth. NHWD-870 (0-50 nM; 24 hours) inhibits BRD4 phosphorylation and c-MYC expression in H526, A2780, ES-2, and MDA-MB231 cells[1].
In vivo	NHWD-870 (0.75-3 mg/kg; p.o.) reduces tumor-associated macrophages (TAMs) in subcutaneous H526 and A2780 tumors by downregulating CSF1 expression in tumor cells, thereby inhibiting TAM proliferation[1].

Chemical Properties

Molecular Weight	491.59
Formula	C₂₉H₂₉N₇O
Cas No.	2115742-03-3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 60 mg/mL (122.05 mM), Sonication and heating to 60℃ are recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0342 mL	10.1711 mL	20.3422 mL	101.7108 mL
5 mM	0.4068 mL	2.0342 mL	4.0684 mL	20.3422 mL
10 mM	0.2034 mL	1.0171 mL	2.0342 mL	10.1711 mL
20 mM	0.1017 mL	0.5086 mL	1.0171 mL	5.0855 mL
50 mM	0.0407 mL	0.2034 mL	0.4068 mL	2.0342 mL
100 mM	0.0203 mL	0.1017 mL	0.2034 mL	1.0171 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc