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NHWD-870

Catalog No. T36573Cas No. 2115742-03-3

NHWD-870 is an effective and selective inhibitor of BET family bromodomain only binding to BRD2, BRD3, BRD4 (IC50 = 2.7 nM), and BRDT. NHWD-870 exhibits potent anti-tumor efficacies and suppresses cancer cell-macrophage interaction through the increase of tumor apoptosis and inhibition of tumor proliferation.

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Pack Size	Price	Availability
25 mg	$1,630	8-10 weeks
50 mg	$2,120	8-10 weeks
100 mg	$3,100	8-10 weeks

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Product Introduction

Bioactivity

Description	NHWD-870 is an effective and selective inhibitor of BET family bromodomain only binding to BRD2, BRD3, BRD4 (IC50 = 2.7 nM), and BRDT. NHWD-870 exhibits potent anti-tumor efficacies and suppresses cancer cell-macrophage interaction through the increase of tumor apoptosis and inhibition of tumor proliferation.
In vitro	NHWD-870 exhibits mild inhibition of the hERG channel (IC50 = 5.4 µM). NHWD-870 shows robust activities inducing apoptosis and suppressing cell proliferation. NHWD-870 (0.01-10000 nM) inhibits melanoma cells (A375) with an IC50 of 2.46 nM. In H526, A2780, ES-2, and MDA-MB231 cells, NHWD-870 (0-10000 nM; 5 days) suppressed cell growth. NHWD-870 (0-50 nM; 24 hours) inhibits BRD4 phosphorylation and c-MYC expression in H526, A2780, ES-2, and MDA-MB231 cells[1].
In vivo	NHWD-870 (0.75-3 mg/kg; p.o.) reduces the number of tumor associated macrophages (TAMs) in subcutaneously implanted H526 and A2780 tumors. NHWD-870 downregulated CSF1 expression in tumor cells to inhibit TAM proliferation[1].

Chemical Properties

Molecular Weight	491.59
Formula	C29H29N7O
Cas No.	2115742-03-3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 60 mg/mL (122.05 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0342 mL	10.1711 mL	20.3422 mL	101.7108 mL
5 mM	0.4068 mL	2.0342 mL	4.0684 mL	20.3422 mL
10 mM	0.2034 mL	1.0171 mL	2.0342 mL	10.1711 mL
20 mM	0.1017 mL	0.5086 mL	1.0171 mL	5.0855 mL
50 mM	0.0407 mL	0.2034 mL	0.4068 mL	2.0342 mL
100 mM	0.0203 mL	0.1017 mL	0.2034 mL	1.0171 mL

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DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

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