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SH5-07

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Purity:95.54%

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COA HNMR HPLC

SH5-07

Catalog No. TQ0052Cas No. 1456632-41-9

SH5-07 is a hydroxamic acid-based Stat3 inhibitor (IC50: 3.9 μM).

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Pack Size	Price	Availability
1 mg	$51	In Stock
2 mg	$74	In Stock
5 mg	$122	In Stock
10 mg	$198	In Stock
25 mg	$347	In Stock
50 mg	$515	In Stock
100 mg	$747	In Stock
1 mL x 10 mM (in DMSO)	$169	In Stock

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Product Introduction

Bioactivity

Description	SH5-07 is a hydroxamic acid-based Stat3 inhibitor (IC50: 3.9 μM).
Targets&IC50	STAT3:3.9 μM (cell free)
In vitro	SH5-07, a hydroxamic acid analog of BP-1-102, selectively inhibits Stat3 activity in a dose-dependent manner (IC50: 3.9±0.6 μM in vitro), specifically targeting Stat3:Stat3 DNA-binding over Stat1:Stat3, with minimal impact on Stat1:Stat1. By binding to Stat3, it disrupts its interaction with growth factor receptors, thus inhibiting Stat3 phosphorylation. This inhibition leads to reduced expression of Stat3-regulated genes, including Bcl-xL, Bcl-2, c-Myc, Survivin, Cyclin D1, and Mcl-1, following a 24-hour exposure to 5 μM SH5-07, ultimately exerting antitumor effects against cells with constitutively active Stat3.
In vivo	Tail vein injection or oral gavage delivery of SH5-07 inhibits the growth of 90-150 mm^3 established subcutaneous mouse xenografts of human glioma (U251MG) and breast (MDA-MB-231) tumors with aberrantly-active Stat3, associated with decreased Mcl-1, c-Myc, and Cyclin D1 expression. No significant changes in body weights, blood cell counts, organ gross anatomy, or signs of toxicity are observed.
Cell Research	Cells are treated with 0-8 μM agent for 24-48 h. For cell cycle profile analysis, cells are harvested and fixed with 70% ice-cold ethanol and stained with propidium iodide (PI). For apoptosis analysis, cells are collected and stained with FITC-Annexin V using the Apoptosis Detection Kit. Both the DNA content of cells and the Annexin V-positive cells are analyzed by the flow cytometer. Cell cycle phase distribution is analyzed using the Cell-Fit program. Data acquisition is gated to exclude cell doublets.
Animal Research	Mice are injected subcutaneously in the left flank area with U251MG cells in 200 μL of PBS/Matrigel matrix, or MDA-MB-231 cells in 100 μL of PBS. Mice with tumors of 90-150 mm^3 (MDA-MB-231) or 150 mm^3 (U251MG) are grouped for identical mean tumor sizes, administered 3, 5 or 6 mg/kg SH5-07 via oral gavage daily or tail vein injection every 2 or 3 days, and monitored every 3-7 days. Tumor sizes are measured with calipers and converted to tumor volume.

Chemical Properties

Molecular Weight	625.61
Formula	C29H28F5N3O5S
Cas No.	1456632-41-9

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 50 mg/mL (79.92 mM), Sonication is recommended.

H2O: Insoluble

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.5984 mL	7.9922 mL	15.9844 mL	79.9220 mL
5 mM	0.3197 mL	1.5984 mL	3.1969 mL	15.9844 mL
10 mM	0.1598 mL	0.7992 mL	1.5984 mL	7.9922 mL
20 mM	0.0799 mL	0.3996 mL	0.7992 mL	3.9961 mL
50 mM	0.0320 mL	0.1598 mL	0.3197 mL	1.5984 mL

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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

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mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

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