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6-Hydroxyflavone

Catalog No. T2940   CAS 6665-83-4
Synonyms: 6-HF

6-Hydroxyflavone (6-HF) is a noncompetitive inhibitors of cytochrome P450 2C9. It is a flavone, a type of chemical compound. It is reported in leaves of Barleria prionitis Linn. (a common Acanthaceae from India). 6-Hydroxyflavone may have a potential as a therapeutic drug capable for the treatment of anxiety-like disorders.

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6-Hydroxyflavone Chemical Structure
6-Hydroxyflavone, CAS 6665-83-4
Pack Size Availability Price/USD Quantity
100 mg In stock $ 39.00
500 mg In stock $ 91.00
1 mL * 10 mM (in DMSO) In stock $ 43.00
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Purity: 99.83%
Purity: 99.73%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description 6-Hydroxyflavone (6-HF) is a noncompetitive inhibitors of cytochrome P450 2C9. It is a flavone, a type of chemical compound. It is reported in leaves of Barleria prionitis Linn. (a common Acanthaceae from India). 6-Hydroxyflavone may have a potential as a therapeutic drug capable for the treatment of anxiety-like disorders.
Kinase Assay For Jak3 kinase assays, Fsk-treated MT-2 cells are lysed, clarified, and immunoprecipitated using Jak3 antibody. Kinase reactions are carried out at 30°C for 20 min. For PKA kinase assays, untreated MT-2 cells are lysed, and Jak3 is immunoprecipitated and bound to PAS beads. Immunoprecipitated Jak3 is washed with kinase buffer (50 mM Hepes-NaOH (pH 7.4), 10 mM MgCl2, 0.5 mM EGTA, 0.5 mM DTT, 20 μg/mL aprotinin, 10 μg/mL leupeptin, 1 μg/mL pepstatin A) and incubated with 200 μM ATP and purified protein kinase A catalytic subunit (PKAc) as indicated in the figure legends. Kinase reactions are carried out at 32 °C for 30 min followed by vigorous washing of the beads with cold kinase wash buffer. For [γ-32P]ATP radiolabeled kinase assays using recombinant Jak3, Hek293 cells are transfected with wild type (WT) Jak3 or kinase-dead Jak3 K855A using Lipofectamine 2000 according to the manufacturer's instructions. Cells are lysed and immunoprecipitated with Jak3 antibody. Jak3-bound PAS beads are washed three times in cold lysis buffer followed by kinase buffer. Kinase reactions are initiated by adding 10 μCi [γ-32P]ATP, 10 μM unlabeled ATP, and 1 μg of purified PKAc to Jak3-bound PAS bead reaction mixtures. Kinase reactions are performed at 32°C for 30 min. Jak3-bound PAS beads are washed three times in radioimmunoassay buffer (10 mM Tris-HCl, pH 7.4, 75 mM NaCl, 20 mM EDTA, 10 mM EGTA, 20 mM Na4P2O7, 50 mM NaF, 20 mM 2-glycerolphosphate, 1 mM p-nitrophenylphosphate, 0.1% Triton X-100) and one time in kinase wash buffer. The reactions are stopped by adding 2× SDS-PAGE sample buffer followed by SDS-PAGE. Coomassie stainable Jak3 bands are excised from the PVDF membrane and subjected to phosphoamino acid analysis[2].
Synonyms 6-HF
Molecular Weight 238.24
Formula C15H10O3
CAS No. 6665-83-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 6.5 mg/mL (27.28 mM), Sonification is recommended

TargetMolReferences and Literature

1. Wang X, et al. PLoS One. 2015 Mar 19;10(3):e0116409.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Neurodegenerative Disease Compound Library Anti-Aging Compound Library Natural Product Library for HTS Anti-Parkinson's Disease Compound Library Bioactive Compound Library Flavonoid Natural Product Library Selected Plant-Sourced Compound Library Inhibitor Library Neurotransmitter Receptor Compound Library Neuronal Signaling Compound Library

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Keywords

6-Hydroxyflavone 6665-83-4 Membrane transporter/Ion channel Neuroscience GABA Receptor inhibit Inhibitor 6-HF 6 Hydroxyflavone 6Hydroxyflavone inhibitor

 

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