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Dasabuvir

Catalog No. T3489   CAS 1132935-63-7
Synonyms: ABT-333

Dasabuvir (ABT-333) is a non-nucleoside inhibitor of the hepatitis C virus (HCV) non-structural protein 5B (NS5B), an RNA-dependent RNA polymerase, with potential activity against HCV. Upon administration and after intracellular uptake, dasabuvir binds HCV NS5B polymerase and blocks viral RNA synthesis and replication. The HCV NS5B protein is essential for the replication of the HCV RNA genome.

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Dasabuvir Chemical Structure
Dasabuvir, CAS 1132935-63-7
Pack Size Availability Price/USD Quantity
2 mg In stock $ 33.00
5 mg In stock $ 52.00
10 mg In stock $ 77.00
25 mg In stock $ 139.00
50 mg In stock $ 223.00
100 mg In stock $ 369.00
1 mL * 10 mM (in DMSO) In stock $ 57.00
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Purity: 99.62%
Purity: 99.54%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Dasabuvir (ABT-333) is a non-nucleoside inhibitor of the hepatitis C virus (HCV) non-structural protein 5B (NS5B), an RNA-dependent RNA polymerase, with potential activity against HCV. Upon administration and after intracellular uptake, dasabuvir binds HCV NS5B polymerase and blocks viral RNA synthesis and replication. The HCV NS5B protein is essential for the replication of the HCV RNA genome.
Targets&IC50 GnRH:0.33 nM
In vitro ABT-333 (Dasabuvir) is at least 7,000-fold selective for the inhibition of HCV genotype 1 polymerases over human/mammalian polymerases. ABT-333 (Dasabuvir) inhibits the polymerase enzymatic activity of genotype 1 laboratory strain enzymes (H77, BK, N, and Con1 strains), as well as enzymes produced from polymerase genes from HCV genotype 1-infected subjects, with IC50s between 2.2 and 10.7 nM. ABT-333 inhibits replication of HCV subgenomic replicons in cell culture assays, with EC50 values of 7.7 and 1.8 nM against genotype 1a (H77) and 1b (Con1), respectively. In the presence of 40% human plasma, there is a 12- to 13-fold decrease in inhibitory potency, yielding EC50s of 99 and 21 nM for HCV genotype 1a (H77) and 1b (Con1) replicons, respectively[1].
Kinase Assay The recombinant HCV polymerases used in this study contain the first 570 amino acids of the 591-amino acid native protein sequence, with a six-histidine tag at the N terminus to facilitate purification by affinity chromatography. Briefly, ABT-333 (Dasabuvir) is incubated with 5 to 50 nM polymerase for 15 min at room temperature, follow by the addition of nucleoside triphosphates (NTPs) and [3H]UTP for 3 h at 30°C. After termination of the reaction, the precipitated RNA is captured by filtration through a GF/B filter. The amount of incorporated [3H]UTP is measured by scintillation counting with a Wallac 1450 MicroBeta counter. The percent inhibition is calculated from the initial rates of inhibited reactions relative to that of the uninhibited control reaction. The mean 50% inhibitory concentration (IC50) and the standard error of the mean (SEM) are calculated via nonlinear regression[1].
Synonyms ABT-333
Molecular Weight 493.57
Formula C26H27N3O5S
CAS No. 1132935-63-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 46 mg/mL

TargetMolReferences and Literature

1. Kati W, et al. In vitro activity and resistance profile of dasabuvir, a nonnucleoside hepatitis C virus polymerase inhibitor. Antimicrob Agents Chemother. 2015 Mar;59(3):1505-11.

TargetMolCitations

1. Hayashi T, Murakami K, Hirano J, et al. Dasabuvir inhibits human norovirus infection in human intestinal enteroids. mSphere. 2021, 6(6): e00623-21.

Related compound libraries

This product is contained In the following compound libraries:
Highly Selective Inhibitor Library NO PAINS Compound Library Nucleotide Compound Library Bioactive Compound Library Drug Repurposing Compound Library Anti-COVID-19 Compound Library Bioactive Compounds Library Max Inhibitor Library Pediatric Drug Library Anti-Viral Compound Library

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Keywords

Dasabuvir 1132935-63-7 Microbiology/Virology Proteases/Proteasome HCV Protease ABT-333 Hepatitis C virus ABT333 genotype Inhibitor DNA/RNA Synthesis RNA HCV inhibit HCV polymerase HCV genotype 1 infections ABT 333 inhibitor

 

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