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SL327

Catalog No. T2708Cas No. 305350-87-2
Alias SL-327, SL 327

SL327 is a selective inhibitor for MEK1/2 with IC50 of 0.18 μM/0.22 μM; able to transport through the blood-brain barrier.

SL327

SL327

Purity: 100%
Catalog No. T2708Alias SL-327, SL 327Cas No. 305350-87-2
SL327 is a selective inhibitor for MEK1/2 with IC50 of 0.18 μM/0.22 μM; able to transport through the blood-brain barrier.
Pack SizePriceAvailabilityQuantity
5 mg$31In Stock
10 mg$40In Stock
25 mg$64In Stock
50 mg$98In Stock
100 mg$163In Stock
1 mL x 10 mM (in DMSO)$35In Stock
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Purity:100%
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Product Introduction

Bioactivity
Description
SL327 is a selective inhibitor for MEK1/2 with IC50 of 0.18 μM/0.22 μM; able to transport through the blood-brain barrier.
Targets&IC50
MEK1:0.18 μM, MEK2:0.22 μM
In vitro
Administration of 30 mg/kg of SL327 significantly impairs spatial learning and memory in mice. At a higher dose of 50 mg/kg, SL327 can cross the blood-brain barrier and inhibit conditioned fear by suppressing the phosphorylation of MAPK/ERK.
In vivo
SL327 does not inhibit a variety of other kinases, including PKA, PKC, or CamKII.
Kinase Assay
The ligand binding competition assays are performed. Cytosolic cell extracts from Hepa-1 cells are generated by the resuspension of the cell pellets in HEDG buffer [25 mM Hepes, 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol, pH 7.5] containing 0.4 mM leupeptin, 4 mg/mL aprotinin, and 0.3 mM phenylmethylsulfonyl fluoride, homogenization, and centrifugation at 100,000 g for 45 min. Aliquots of the supernatant (120 μg) are incubated at room temperature for 2 h with the indicated concentrations of Pifithrin-α in the presence of 3 nM [3H]TCDD in HEDG buffer. After incubation on ice with hydroxyapatite for 30 min, HEDG buffer with 0.5% Tween 80 is added. The samples are centrifuged, washed twice, resuspended in 0.2 mL of scintillation fluid, and subjected to scintillation counting. Nonspecific binding is determined using a 150-fold molar excess of TCDF and subtracted from the total binding to obtain the specific binding. The specific binding is reported relative to [3H]TCDD alone[2].
AliasSL-327, SL 327
Chemical Properties
Molecular Weight335.35
FormulaC16H12F3N3S
Cas No.305350-87-2
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 50 mg/mL (149.1 mM), Sonication is recommended.
Ethanol: 16.8 mg/mL (50 mM)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM2.9820 mL14.9098 mL29.8196 mL149.0980 mL
5 mM0.5964 mL2.9820 mL5.9639 mL29.8196 mL
10 mM0.2982 mL1.4910 mL2.9820 mL14.9098 mL
20 mM0.1491 mL0.7455 mL1.4910 mL7.4549 mL
50 mM0.0596 mL0.2982 mL0.5964 mL2.9820 mL
DMSO
1mg5mg10mg50mg
100 mM0.0298 mL0.1491 mL0.2982 mL1.4910 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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