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Wnt-C59

Catalog No. T2242   CAS 1243243-89-1
Synonyms: C59

Wnt-C59 (C59)(C59) is a highly effective and specific Wnt signaling antagonis with PORCN enzymatic activity.

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Wnt-C59 Chemical Structure
Wnt-C59, CAS 1243243-89-1
Pack Size Availability Price/USD Quantity
2 mg In stock $ 33.00
5 mg In stock $ 48.00
10 mg In stock $ 75.00
25 mg In stock $ 132.00
50 mg In stock $ 210.00
100 mg In stock $ 360.00
1 mL * 10 mM (in DMSO) In stock $ 53.00
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Purity: 99.91%
Purity: 99.56%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Wnt-C59 (C59)(C59) is a highly effective and specific Wnt signaling antagonis with PORCN enzymatic activity.
Targets&IC50 Porcn:74 pM
Kinase Assay Aurora A radioactive Flashplate enzyme assay: Aurora A radioactive Flashplate enzyme assay is conducted to determine the nature and degree of MLN8237-mediated inhibition in vitro. Recombinant Aurora A is expressed in Sf9 cells and purified with GST affinity chromatography. The peptide substrate for Aurora A is conjugated with biotin (Biotin-GLRRASLG). Aurora A kinase (5 nM) is assayed in 50 mM Hepes (pH 7.5), 10 mM MgCl2, 5 mM DTT, 0.05% Tween 20, 2 μM peptide substrate, 3.3 μCi/mL [γ-33P]ATP at 2 μM, and increasing concentrations of MLN8237 by using Image FlashPlates.
Cell Research Wnt-C59 (C59) is dissolved in DMSO (10 mM) and stored, and then diluted with appropriate medium before use[1]. Approximately, 1×104 cells are seeded in 24-well plates, and Wnt-C59 (5 μM, 10 μM, and 20 μM) is added the next day. Each group is tested in triplicate and control groups with addition of DMSO are also established. Cell confluence is determined by microscopy at 24, 48, 72, and 96 hours after seeding of cells. The IC50 of Wnt-C59 is determined by MTT assay, using 96-well dishes. Next day, various concentrations of Wnt-C59 are added, and cellular viabilities are measured by a spectrophotometer at both 24 and 48 hours. For sphere formation, approximately one hundred cells are seeded onto the Low Cell Bind Surface 24-well Nunc dish. Each group is done in triplicate and each well had 2 mL medium. Media are changed twice a week, and only half of the media is changed each time. Approximately, 1×103 cells are seeded for each well in the sphere inhibition assay. At 1 to 5 days after plating, all tested cells formed small spheres. Five days later, Wnt-C59 (1 μM, 5 μM, and 20 μM) is added into experimental groups. Abilities for cell growth and sphere images are compared and recorded at the end of the first, second, and third weeks after addition of Wnt-C59, or DMSO in control groups. The sphere growths are observed and recorded daily under microscopy, and the area of spheres is analyzed using Metamorph and recorded as average area (μm2)[1].
Synonyms C59
Molecular Weight 379.45
Formula C25H21N3O
CAS No. 1243243-89-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 7.6 mg/mL (20 mM)

DMSO: 7.6 mg/mL (20 mM)

TargetMolReferences and Literature

1. Proffitt KD, et al. Cancer Res, 2013, 73(2), 502-507. 2. Wend P, et al. EMBO Mol Med, 2013, 5(2), 264-279. 3. Petrova E, et al. Nat Chem Biol, 2013, 9(4), 247-249. 4. Youssef KK, et al. Nat Cell Biol, 2012, 14(12), 1282-1294.

TargetMolCitations

1. Zhang, Linhao, et al. Inhibition of cyclooxygenase‐2 enhanced intestinal epithelial homeostasis via suppressing β‐catenin signalling pathway in experimental liver fibrosis. Journal of Cellular and Molecular Medicine. 2021

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compounds Library Max Osteogenesis Compound Library Anti-Obesity Compound Library Inhibitor Library Anti-Cancer Compound Library Anti-Cancer Metabolism Compound Library Anti-Aging Compound Library NO PAINS Compound Library Anti-Prostate Cancer Compound Library Orally Active Compound Library

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Related compounds with same targets
β-catenin-IN-2 Cardiogenol C hydrochloride TNIK-IN-2 KY1220 Bisdemethoxycurcumin Laduviglusib trihydrochloride Ginkgetin Wnt/β-catenin agonist 3

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Keywords

Wnt-C59 1243243-89-1 Cytoskeletal Signaling Stem Cells Porcupine Wnt/beta-catenin Wnt WntC59 Inhibitor C59 Wnt-C-59 inhibit C 59 C-59 Wnt C59 inhibitor

 

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