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Z-FA-FMK

Catalog No. T6738 CAS 197855-65-5

Z-FA-FMK can irreversibly inhibit cysteine protease and also inhibit effector caspases.

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Z-FA-FMK, CAS 197855-65-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 61.00
5 mg	In stock	$ 198.00
10 mg	In stock	$ 297.00
25 mg	In stock	$ 479.00
50 mg	In stock	$ 653.00
100 mg	In stock	$ 929.00
1 mL * 10 mM (in DMSO)	In stock	$ 178.00

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Purity: 99.68%

Purity: 99.43%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Z-FA-FMK can irreversibly inhibit cysteine protease and also inhibit effector caspases.
Targets&IC50	3CLpro (SARS Protease):25.7 μM (Ki)
In vitro	Z-FA-FMK inhibits the development of Ras-induced carcinogenic tumors and respiratory viral infections in the cardiac tissue of mice with severe combined immunodeficiency (SCID). In a mouse model of intranasal pneumococcal infection, Z-FA-FMK significantly promotes the growth of Streptococcus pneumoniae in both the lungs and the bloodstream.
In vivo	Z-FA-FMK effectively inhibits T-cell proliferation induced by interleukin-2 (IL-2) and mitogens in vitro. It also prevents the degradation of fibrillar collagen through its actions on fibroblasts and osteoclasts. Furthermore, Z-FA-FMK suppresses the expression of NF-κB-dependent genes in macrophages, thereby inhibiting the production of cytokines induced by lipopolysaccharides.
Cell Research	T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester. (Only for Reference)

Molecular Weight	386.42
Formula	C21H23FN2O4
CAS No.	197855-65-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 71 mg/mL (183.7 mM)

Ethanol: 32 mg/mL (82.8 mM)

References and Literature

1. Rasnick D. Anal Biochem. 1985, 149(2), 461-465. 2. van Noorden CJ, et al. Biochem Biophys Res Commun. 1991, 178(1), 178-184. 3. Schotte P, et al. J Biol Chem. 2001, 276(24), 21153-21157. 4. Lawrence CP, et al. J Immunol. 2006, 177(6), 3827-3836. 5. Kim M, et al. Antivir Ther. 2010, 15(6), 897-905.

Related compound libraries

This product is contained In the following compound libraries：

Highly Selective Inhibitor Library Inhibitor Library Bioactive Compounds Library Max Covalent Inhibitor Library Anti-Aging Compound Library Target-Focused Phenotypic Screening Library NO PAINS Compound Library Fluorochemical Library Apoptosis Compound Library Anti-Viral Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Z-FA-FMK 197855-65-5 Microbiology/Virology Proteases/Proteasome Cysteine Protease SARS-CoV SARS coronavirus RRMs DNA fragmentation DEVDase Caspase Cathepsin Inhibitor externalization of phosphatidylserine Apoptosis inhibit Z FA FMK reovirus replication ZFAFMK inhibitor

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