Shopping Cart
- Remove All
Your shopping cart is currently empty
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 mg | $30 | In Stock | |
50 mg | $40 | In Stock | |
100 mg | $56 | In Stock | |
200 mg | $81 | In Stock | |
500 mg | $133 | In Stock | |
1 g | $198 | In Stock | |
1 mL x 10 mM (in DMSO) | $39 | In Stock |
Description | Azathramycin (Desmethyl Azithromycin) is an antibiotic derived from erythromycin, binding to the 50S subunit of the bacterial ribosome, and thus inhibits translation of mRNA. |
Cell Research | THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm. |
Alias | Desmethyl Azithromycin, 6-Demethylazithromycin, Azaerythromycin A, Azaerythromycin |
Molecular Weight | 734.96 |
Formula | C37H70N2O12 |
Cas No. | 76801-85-9 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | |||||||||||||||
Solubility Information | DMSO: 7.35 mg/mL (10 mM) | |||||||||||||||
Solution Preparation Table | ||||||||||||||||
DMSO
|
Copyright © 2015-2024 TargetMol Chemicals Inc. All Rights Reserved.