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FLI-06

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Purity:100%

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COA HPLC HNMR

FLI-06

Catalog No. T3075Cas No. 313967-18-9

FLI-06 is a Notch signaling inhibitor (EC50: 2.3 μM).

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Pack Size	Price	Availability
1 mg	$30	In Stock
2 mg	$44	In Stock
5 mg	$68	In Stock
10 mg	$101	In Stock
25 mg	$181	In Stock
50 mg	$328	In Stock
100 mg	$571	In Stock
500 mg	$1,250	In Stock
1 mL x 10 mM (in DMSO)	$75	In Stock

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Product Introduction

Bioactivity

Description	FLI-06 is a Notch signaling inhibitor (EC50: 2.3 μM).
In vitro	In zebrafish embryos, FLI-06 at a concentration of 50µM inhibits the endogenous Notch signaling pathway.
In vivo	In kidney epithelial 293 cells, stable transfection of mutant APP results in significant amyloid deposition. FLI-06 alters the maturation pattern of the APP, subsequently terminating the release of APPs. Furthermore, in Hela cells overexpressing Notch green fluorescent protein, FLI-06 inhibits Notch transport and progression. FLI-06 disrupts the Golgi apparatus and inhibits the secretion of pre-transport proteins in the endoplasmic reticulum during the tubular to sheet-like morphology transition.
Kinase Assay	EC50 values of the test compounds are calculated from serial dilution series ranging from 200 to 0.1 μM. Cells are seeded in 96-well plates at a density of 5,000 cells per well in 100 μL medium. The next day, 100 μL medium containing each test compound is added. Cells are incubated for 16 h, fixed and processed for automated microscopy. EC50 estimates are calculated using four-parameter log-logistic fit with the package drc[1].
Alias	FLI 06

Chemical Properties

Molecular Weight	438.52
Formula	C25H30N2O5
Cas No.	313967-18-9

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 8.8 mg/mL (20 mM)

DMSO: 60 mg/mL (136.82 mM)

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.2804 mL	11.4020 mL	22.8040 mL	114.0199 mL
5 mM	0.4561 mL	2.2804 mL	4.5608 mL	22.8040 mL
10 mM	0.2280 mL	1.1402 mL	2.2804 mL	11.4020 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1140 mL	0.5701 mL	1.1402 mL	5.7010 mL
50 mM	0.0456 mL	0.2280 mL	0.4561 mL	2.2804 mL
100 mM	0.0228 mL	0.1140 mL	0.2280 mL	1.1402 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc