Hesperidin (Cirantin) is a flavanone glycoside found in citrus fruits with antioxidant, anti-inflammatory, anti-carcinogenic, and antihypertensive and lipid-lowering activity.

Anti-hypertensive agent with lipid-lowering:116.68μmo/L

METHODS: Tumor cells HeLa and HT-29 were treated with Hesperidin (20-100 µM) for 24-72 h. Cell viability was measured by MTT assay.
RESULTS: Treatment with Hesperidin significantly reduced cell viability and the effect of Hesperidin on cell viability was concentration and time dependent. Cells incubated with 100 µM Hesperidin for 72 h showed the greatest antiproliferative effect, with cell viability decreasing to 12% of control cells. [1]
METHODS: Human mesothelioma cells MSTO-211H were treated with Hesperidin (40-160 µM) for 48 h. Apoptosis was detected using DAPI and PI staining.
RESULTS: The percentage of sub-G1 phase increased from 20% to 35% in 40-160 µM Hesperidi-treated MSTO-211H cells. [2]

METHODS: To assay antitumor activity in vivo, Hesperidin (200 mg/kg) was administered by gavage to BALB/c mice bearing CT-26 xenografts once daily for five days. Hesperidin was then administered as a single dose of cyclophosphamide (25 mg/kg) for fourteen days.
RESULTS: Leukocyte counts were increased in mice treated with Hesperidin prior to cyclophosphamide injection. This significant protective effect was observed 4 and 7 days after cyclophosphamide injection. In CT-26 tumor-bearing mice, co-administration of Hesperidin and cyclophosphamide significantly inhibited cyclophosphamide-induced delay in tumor growth. [3]