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KNK437

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Catalog No. T2444Cas No. 218924-25-5
Alias Heat Shock Protein Inhibitor I

KNK437 (Heat Shock Protein Inhibitor I), a pan-HSP inhibitor, suppresses the synthesis of inducible HSPs(HSP105, HSP72, and HSP40).

KNK437

KNK437

🥰Excellent
Purity: 98%
Catalog No. T2444Alias Heat Shock Protein Inhibitor ICas No. 218924-25-5
KNK437 (Heat Shock Protein Inhibitor I), a pan-HSP inhibitor, suppresses the synthesis of inducible HSPs(HSP105, HSP72, and HSP40).
Pack SizePriceAvailabilityQuantity
5 mg49 €In Stock
10 mg70 €In Stock
25 mg152 €In Stock
50 mg277 €In Stock
100 mg521 €In Stock
200 mg718 €In Stock
1 mL x 10 mM (in DMSO)54 €In Stock
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Purity:98%
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Product Introduction

Bioactivity
Description
KNK437 (Heat Shock Protein Inhibitor I), a pan-HSP inhibitor, suppresses the synthesis of inducible HSPs(HSP105, HSP72, and HSP40).
In vivo
KNK437 (200 mg/kg, i.p.) shows no antitumor effects and does not increase the thermosensitivity of nontolerant tumors. The same dose of KNK437 enhances the antitumor effects of fractionated heat treatment in a synergistic manner.[3]
Kinase Assay
Metabolic Labeling and Gel Electrophoresis: COLO 320DM cells (200,000) are injected into each well of 12-well plastic plates 2 days before incubation in the presence of KNK437 for 1 h before heat shock. The cells are then heat-shocked at 42°C for 90 min or kept at 37°C for the same length of time and incubated at 37°C for 2 h. For metabolic labeling, cells are washed with PBS without Ca2+ or Mg2+ and incubated for 1 h with 1.22 MBq of [35S]methionine in 250 μL of methionine-free DMEM supplemented with 10% dialyzed fetal bovine serum. After metabolic labeling, cells are washed twice with PBS and lysed in a buffer containing 1% NP40, 0.15 M NaCl, 50 mM Tris-HCl (pH 8.0), 5 mM EDTA, and protease inhibitors [0.2 mM 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, 2 mM N-ethylmaleimide, 1 μg/mL pepstatin, and 1 μg/mL leupeptin]. After centrifugation at 12,000×g for 20 min, cell extracts containing equal amounts of trichloroacetic acid-insoluble radioactivity are analyzed by two-dimensional gel electrophoresis (the one-dimensional gel electrophoresis is a nonequilibrium pH gradient gel electrophoresis, and the two-dimensional gel electrophoresis is 10% SDS-PAGE).
Cell Research
Thermotolerance is induced by incubating cells with 300 μM sodium arsenite for 90 min. Cells are preincubated with or without 100 μM KNK437 for 1 h before the sodium arsenite treatment. After treatment of the cells with sodium arsenite, cells are washed once with PBS and incubated at 37℃ for 5 h with or without KNK437. The effects of KNK437 on acquired thermotolerance are tested by heating the cells at 45°C for the indicated time. The surviving fraction is calculated as the plating efficiency of the treated cells divided by the plating efficiency of untreated control cells.(Only for Reference)
AliasHeat Shock Protein Inhibitor I
Chemical Properties
Molecular Weight245.23
FormulaC13H11NO4
Cas No.218924-25-5
SmilesO=CN1CCC(=Cc2ccc3OCOc3c2)C1=O
Relative Density.1.515 g/cm3 (Predicted)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 12 mg/mL (48.9 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM4.0778 mL20.3890 mL40.7780 mL203.8902 mL
5 mM0.8156 mL4.0778 mL8.1556 mL40.7780 mL
10 mM0.4078 mL2.0389 mL4.0778 mL20.3890 mL
20 mM0.2039 mL1.0195 mL2.0389 mL10.1945 mL

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