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PF-06447475

PF-06447475 is a highly effective, specific, brain penetrant LRRK2 inhibitor with IC0 of 3/11 nM for wild type LRRK2 and G2019S LRRK2 respectively.

Catalog No. T2050Cas No. 1527473-33-1

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Purity:99.62%

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COA HPLC HNMR

PF-06447475

Catalog No. T2050Cas No. 1527473-33-1

PF-06447475 is a highly effective, specific, brain penetrant LRRK2 inhibitor with IC0 of 3/11 nM for wild type LRRK2 and G2019S LRRK2 respectively.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Pack Size	Price	Availability
5 mg	$43	In Stock
10 mg	$61	In Stock
25 mg	$89	In Stock
50 mg	$133	In Stock
100 mg	$225	In Stock
200 mg	$397	In Stock
1 mL x 10 mM (in DMSO)	$48	In Stock

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Product Introduction

Bioactivity

Description	PF-06447475 is a highly effective, specific, brain penetrant LRRK2 inhibitor with IC0 of 3/11 nM for wild type LRRK2 and G2019S LRRK2 respectively.
Targets&IC50	LRRK2 (G2019S):11nM, LRRK2:3nM
In vitro	In the macrophage cell line Raw264.7, PF-06447475 inhibits endogenous LRRK2 kinase activity with IC50 of <10 nM. [2] In astrocytes, PF-06447475 rescues LRRK2 mutation-induced defects in lysosomal morphology and function. [3]
In vivo	In G2019S BAC-transgenic mice, PF-06447475 (100 mg/kg, p.o.) inhibits pS935 and pS1292 phosphorylation of LRRK2 with IC50 of 103 nM and 21 nM, respectively. [1] In G2019S-LRRK2 rats, PF-06447475 (30 mg/kg, p.o.) blocks α-synuclein-induced dopaminergic neurodegeneration and attenuates neuroinflammation associated with G2019S-LRRK2 expression. [2]

Chemical Properties

Molecular Weight	305.33
Formula	C₁₇H₁₅N₅O
Cas No.	1527473-33-1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 15.3 mg/mL (50 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2751 mL	16.3757 mL	32.7514 mL	163.7572 mL
5 mM	0.6550 mL	3.2751 mL	6.5503 mL	32.7514 mL
10 mM	0.3275 mL	1.6376 mL	3.2751 mL	16.3757 mL
20 mM	0.1638 mL	0.8188 mL	1.6376 mL	8.1879 mL
50 mM	0.0655 mL	0.3275 mL	0.6550 mL	3.2751 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

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DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

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