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SCR7 pyrazine

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Purity:99.85%

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COA LCMS HNMR

SCR7 pyrazine

Catalog No. T1724Cas No. 14892-97-8

SCR7 pyrazine (SCR7) enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).

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Pack Size	Price	Availability
1 mg	$33	In Stock
2 mg	$46	In Stock
5 mg	$72	In Stock
10 mg	$123	In Stock
25 mg	$271	In Stock
50 mg	$429	In Stock
100 mg	$619	In Stock
1 mL x 10 mM (in DMSO)	$72	In Stock

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Product Introduction

Bioactivity

Description	SCR7 pyrazine (SCR7) enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).
In vitro	SCR7 effectively inhibits the formation of multimers at 200 μM and above. SCR7 successfully inhibits cell proliferation of MCF7, A549, HeLa, T47D, A2780, HT1080, and Nalm6 with IC50 of 40, 34, 44, 8.5, 120, 10, and 50 μM, respectively.[1] SCR7 suppresses the NHEJ repair of CRISPR-Cas9-induced DSBs.[2]
In vivo	SCR7 treatment (10 mg/kg, i.m.) signi?cantly reduces breast adenocarcinoma-induced tumor, and exhibits 4-fold increase in lifespan compared with control group. However, in Swiss albino mice with Dalton's lymphoma tumor model, SCR7 (20 mg/kg, i.p.) exhibits neither tumor regression nor increase in lifespan. In BALB/c mice, SCR7 (20 mg/kg, i.p.) signi?cantly enhances the cytotoxic effects of radiation, etoposide and 3-Aminobenzamide on tumor derived from Dalton's lymphoma (DLA) cells.[1]
Kinase Assay	Complementation of SCR7 Inhibition with Puri?ed Ligase IV: Complementation experiment is carried out by adding increasing concentrations of puri?ed Ligase IV/XRCC4 complex (30, 60, and 120 fmol) along with the oligomeric DNA substrates (5' compatible and 5'-5' noncompatible ends) to the SCR7-treatedextracts. Reactions are incubated for 2 h at 25℃. The reaction products are then resolved on 8% denaturing PAGE. The gel is dried and exposed and the signal is detected with a PhosphorImager and analyzed with Multi Gauge (V3.0) software.
Cell Research	Cell proliferation of cancer cells are determined by MTT and trypan blue assays. Briefly, MCF7, CEM, HeLa, A549, HT1080, A2780, T47D, Nalm6, N114 and K562 cells are grown in presence of SCR7 (10, 50, 100, and 250?μM) for 24 or 48?h, and subjected to MTT or trypan blue assays. Each experiment is repeated a minimum of three independent times. (Only for Reference)
Alias	SCR7

Chemical Properties

Molecular Weight	332.38
Formula	C18H12N4OS
Cas No.	14892-97-8

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 3 mg/mL (9.03 mM)

DMSO: 66 mg/mL (198.57 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	3.0086 mL	15.0430 mL	30.0860 mL	150.4302 mL
5 mM	0.6017 mL	3.0086 mL	6.0172 mL	30.0860 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.3009 mL	1.5043 mL	3.0086 mL	15.0430 mL
20 mM	0.1504 mL	0.7522 mL	1.5043 mL	7.5215 mL
50 mM	0.0602 mL	0.3009 mL	0.6017 mL	3.0086 mL
100 mM	0.0301 mL	0.1504 mL	0.3009 mL	1.5043 mL

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DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

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mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

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