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CCCP

Catalog No. T7081 CAS 555-60-2

Synonyms: Carbonyl Cyanide m-Chlorophenylhydrazone, Carbonyl cyanide 3-chlorophenylhydrazone

CCCP (Carbonyl Cyanide m-Chlorophenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. CCCP inhibits the activation of STING and its downstream signaling molecules TBK1 and IRF3.

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CCCP, CAS 555-60-2

Pack Size	Availability	Price/USD
100 mg	In stock	$ 41.00
200 mg	In stock	$ 58.00
500 mg	In stock	$ 97.00
1 mL * 10 mM (in DMSO)	In stock	$ 45.00

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Purity: 99.64%

Purity: 98.55%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	CCCP (Carbonyl Cyanide m-Chlorophenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. CCCP inhibits the activation of STING and its downstream signaling molecules TBK1 and IRF3.
In vitro	METHODS: Human cervical cancer cells HeLa were treated with CCCP (20 μM) for 30 min, and the effect on the mitochondria-labeled fluorescent dye DiOC6 was observed using live cell fluorescence microscopy. RESULTS: CCCP-induced loss of mitochondrial membrane potential resulted in efflux of DiOC6 from mitochondria into the cytoplasm and subsequent localization to punctate structures. [1] METHODS: Mouse alveolar epithelial cells, MLE-12, were treated with CCCP (10-50 μM) for 2-18 h. The expression levels of the target proteins were examined by Western Blot. RESULTS: CCCP caused a dose- and time-dependent conversion of LC3-I to LC3-II. [2]
In vivo	METHODS: To assay in vivo activity, CCCP (5 mg/kg) was administered as a single intraperitoneal injection to adult and aged C57BL/6J mice, and hearts were collected 12 h later. RESULTS: Under CCCP-treated conditions, more mitochondria bound to autophagic vesicles were found in the hearts of adult but not aged mice. [3] METHODS: To assay in vivo activity, CCCP (1 mg/kg) was administered intraperitoneally to mt-Rosella mice once a day for three days. RESULTS: Mitochondrial fragments were readily visible on the hearts of CCCP-treated mice, and these fragments were trapped in lysosomes for degradation.CCCP induced mitochondrial autophagy. [4]

Synonyms	Carbonyl Cyanide m-Chlorophenylhydrazone, Carbonyl cyanide 3-chlorophenylhydrazone
Molecular Weight	204.62
Formula	C9H5ClN4
CAS No.	555-60-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 60 mg/mL (293.23 mM)

Ethanol: 5 mg/mL

References and Literature

1. Padman BS, et al. The protonophore CCCP interferes with lysosomal degradation of autophagic cargo in yeast and mammalian cells. Autophagy. 2013 Nov 1;9(11):1862-75. 2. Kagan VE, et al. NDPK-D (NM23-H4)-mediated externalization of cardiolipin enables elimination of depolarized mitochondria by mitophagy. Cell Death Differ. 2016 Jul;23(7):1140-51. 3. Hoshino A, et al. Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart. Nat Commun. 2013;4:2308. 4. Kobayashi S, et al. Novel Dual-Fluorescent Mitophagy Reporter Reveals a Reduced Mitophagy Flux in Type 1 Diabetic Mouse Heart. J Am Osteopath Assoc. 2020 Jul 1;120(7):446-455. 5. Zhang Y, Xu X, Hu M, et al. SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy[J]. Cell Death & Differentiation. 2020: 1-15.

Citations

1. Xu Q, Fu Q, Li Z, et al. The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice. Nature Metabolism. 2021: 1-21. 2. Fan Y, Li C, Bai S, et al. NIR‐II Emissive Ru (II) Metallacycle Assisting Fluorescence Imaging and Cancer Therapy. Small. 2022: 2201625 3. Zhang Y, Xu X, Hu M, et al. SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy. Cell Death & Differentiation. 2020: 1-15. 4. Luo P, Yan H, Du J, et al. PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1). Autophagy. 2021 Oct;17(10):3221-3237. 5. Liu C, Wang L, Wang P, et al.The Mechanism of Tigecycline Resistance in Acinetobacter baumannii Revealed by Proteomic and Genomic Analysis.International Journal of Molecular Sciences.2023, 24(10): 8652. 6. Wang K, Zhou Z, Huang L, et al.PINK1 dominated mitochondria associated genes signature predicts abdominal aortic aneurysm with metabolic syndrome.Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease.2024, 1870(2): 166919. 7. Liu C, Liu J, Lu Q, et al.The Mechanism of Tigecycline Resistance in Acinetobacter baumannii under Sub-Minimal Inhibitory Concentrations of Tigecycline.International Journal of Molecular Sciences.2024, 25(3): 1819.

Related compound libraries

This product is contained In the following compound libraries：

Kinase Inhibitor Library Anti-Neurodegenerative Disease Compound Library Bioactive Compound Library Metabolism Compound Library Pyroptosis Compound Library Anti-Parkinson's Disease Compound Library Immuno-Oncology Compound Library Reprogramming Compound Library Anti-Infection Compound Library Neuronal Signaling Compound Library

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Keywords

CCCP 555-60-2 Apoptosis Immunology/Inflammation Metabolism Microbiology/Virology Neuroscience NF-Κb Antibacterial COX Mitochondrial Metabolism STING IFNAR IκB/IKK MPYS Carbonyl Cyanide m-Chlorophenylhydrazone Carbonyl cyanide 3-chlorophenylhydrazone TMEM173 Interferon-alpha/beta receptor Stimulator of Interferon Genes Bacterial ERIS MITA inhibit Inhibitor Interferon-α/β receptor inhibitor

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