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CCT007093

Catalog No. T1927 CAS 176957-55-4

CCT007093 is an effective PPM1D (WIP1) inhibitor (IC50: 8.4 μM).

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CCT007093, CAS 176957-55-4

Pack Size	Availability	Price/USD
5 mg	In stock	$ 39.00
10 mg	In stock	$ 66.00
25 mg	In stock	$ 125.00
50 mg	In stock	$ 226.00
100 mg	In stock	$ 397.00
500 mg	In stock	$ 1,080.00

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Purity: 99.75%

Purity: 99.16%

Purity: 98%

Biological Description

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Storage & Solubility Information

Description	CCT007093 is an effective PPM1D (WIP1) inhibitor (IC50: 8.4 μM).
Targets&IC50	PPM1D:8.4 μM
In vivo	CCT007093, when used in combination with Paclitaxel, synergistically inhibits four Paclitaxel-resistant triple-negative breast cancer cell lines. CCT007093 selectively exhibits significant inhibition on human tumor cell lines over-expressing PPM1D (MCF-7, KPL-1, and MCF-3B). By activating p38 kinase activity, CCT007093 induces cell death. It selectively promotes apoptosis in breast cancer cells and ectopically expressed Wip1 in keratinocytes, while reducing UV-mediated apoptotic responses in Wip1-deficient naked cells and a skin keratinocyte model.
Kinase Assay	In vitro phosphatase assay: Recombinant PPM1D (20-50?pmol) is diluted in Tris buffer (50?mM, pH 8), NaCl (100?mM), β-mercaptoethanol (1?mM) or DTT (1?mM) and treated with MnCl2 (0, 1, 10 and 20?mM) or MgCl2 (0 and 40?mM). Where appropriate, inhibitors of PPM1D (10-50?μM) are added and the assay mix incubated for 30?min at room temperature. Recombinant phospho-P38 (200?pmol) is then added and the mixture incubated at 37°C for 1?h. The reaction is quenched by the addition of excess ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulphate-sample loading buffer and boiling for 5?min at 95°C followed by gel electrophoresis and western blotting.
Cell Research	Cells are transfected with a pSUPER plasmid and an additional plasmid expressing the blasticidin resistance gene (pEFBsd) in a molar ratio of 10:1. Cells are plated in 10?cm plates 24?h after transfection. Blasticidin selection (5?μg/ml) is initiated 48?h post-transfection and replenished every 3 days. Colonies are fixed in methanol and stained with crystal violet after 14 days. Colonies are quantified on a Colcount and the surviving fraction (SF) determined.(Only for Reference)

Molecular Weight	272.39
Formula	C15H12OS2
CAS No.	176957-55-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: Slightly soluble

References and Literature

1. Rayter S, et al. Oncogene. 2008, 27(8), 1036-1044. 2. Bauer JA, et al. Breast Cancer Res. 2010, 12(3), R41. 3. Lee JS, et al. J Dermatol Sci. 2014, 73(2), 125-134.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Glycometabolism Compound Library Apoptosis Compound Library Metabolism Compound Library Phosphatase Inhibitor Library Autophagy Compound Library Lipid Metabolism Compound Library Bioactive Compound Library Bioactive Compounds Library Max

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Keywords

CCT007093 176957-55-4 Apoptosis Autophagy Metabolism Phosphatase inhibit CCT 007093 CCT-007093 Inhibitor inhibitor

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