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Estradiol

Catalog No. T1048 CAS 50-28-2

Synonyms: E2, 17β-Estradiol, 17β-Oestradiol, β-Estradiol

Estradiol (E2) is a naturally occurring steroidal sex hormone that is essential for female fertility and maintenance of secondary sexual characteristics. Estradiol upregulates IL-6 expression through estrogen receptor beta (ERβ).

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Estradiol, CAS 50-28-2

Pack Size	Availability	Price/USD	Quantity
500 mg	In stock	$ 45.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.58%

Purity: 99.5%

Purity: 99.35%

Purity: 99.14%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Estradiol (E2) is a naturally occurring steroidal sex hormone that is essential for female fertility and maintenance of secondary sexual characteristics. Estradiol upregulates IL-6 expression through estrogen receptor beta (ERβ).
In vitro	METHODS: Human lens epithelial cells HLEC were treated with Estradiol (0.001-10 μM) for 24 h. Cell viability was measured using the MTT. RESULTS: The number of viable cells was significantly reduced in the presence of 10 μM Estradiol. [1] METHODS: Human breast cancer cells MB231 were treated with Estradiol (100 nM) for 24 h. Cell motility was detected using scratch/wound healing assay and transwell assay. RESULTS: Estradiol treatment reduced the migration and invasion of MB231 cells. [2]
In vivo	METHODS: To assay in vivo activity, Estradiol (0.2 mg/kg) and progesterone (5-20 mg/kg) were administered intraperitoneally as a single injection to ovariectomized C57BL/6 female mice. RESULTS: Mice receiving Estradiol alone or Estradiol plus 10-20 mg/kg progesterone showed significantly enhanced memory for new objects. [3] METHODS: To assay in vivo activity, Estradiol (0.2 mg/kg) was administered by gavage to ovariectomized C57BL/6 female mice once daily for two weeks. RESULTS: Long-term activation of ERβ reduced hot flash-like symptoms and enhanced spatial and object recognition memory in ovariectomized mice. [4]
Cell Research	Estradiol is dissolved in 0.01% ethanol. Briefly, B9 cells (5×103/well of a 96-well plate) are cultured with a series of dilutions of the supernatants in a final volume of 100 mL of RPMI 1640, supplemented with 5×10-5mol/Lof 2-mercaptoethanol, 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin in flat-bottom microtiter plates. After 42 h, 0.5 μCi of [3H]thymidine is added. 6 h later, the cells are harvested and the radioactivity incorporated is determined. IL-6 is quantitated from a standard curve set up with known amounts of recombinant human or mouse IL-6. The anti-IL-6 monoclonal antibody completely inhibited the ability of the B9 cells to proliferate in response to recombinant mouse IL-6. In addition, we determined that B9 cells do not proliferate in response to IL-I or TNF, and that antibodies to these two cytokines did not affect the cell proliferation in response to IL-6. Further, it is established that neither Estradiol (17β-estradiol), nor the 0.01% ethanol used as vehicle in these experiments, has any effect on the bioassay.

Synonyms	E2, 17β-Estradiol, 17β-Oestradiol, β-Estradiol
Molecular Weight	272.38
Formula	C18H24O2
CAS No.	50-28-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 20.4 mg/mL (75 mM)

DMSO: 27.2 mg/mL (100 mM)

References and Literature

1. Celojevic D, et al. Effects of 17β-estradiol on proliferation, cell viability and intracellular redox status in native human lens epithelial cells. Mol Vis. 2011;17:1987-96. Epub 2011 Jul 20. 2. Zhao Z, et al. 17β-Estradiol treatment inhibits breast cell proliferation, migration and invasion by decreasing MALAT-1 RNA level. Biochem Biophys Res Commun. 2014 Mar 7;445(2):388-93. 3. Harburger LL, et al. Dose-dependent effects of post-training estradiol plus progesterone treatment on object memory consolidation and hippocampal extracellular signal-regulated kinase activation in young ovariectomized mice. Neuroscience. 2009 Apr 21;160(1):6-12. 4. Fleischer AW, et al. Long-term oral administration of a novel estrogen receptor beta agonist enhances memory and alleviates drug-induced vasodilation in young ovariectomized mice. Horm Behav. 2021 Apr;130:104948. 5. Woolley CS, et al. J Comp Neurol, 1993, 336(2), 293-306. 6. Wang Y, Xu G, Wang Z, et al. Psoralidin, a major component of Psoraleae Fructus, induces inflammasome activation and idiosyncratic liver injury[J]. International Immunopharmacology. 2021, 92: 107352. 7. Zhang S, Sun C, Zhao S, et al. Exposure to DEHP or its metabolite MEHP promotes progesterone secretion and inhibits proliferation in mouse placenta or JEG-3 cells[J]. Environmental Pollution. 2020, 257: 113593. 8. Qiao L, Wang H, He J, et al. Truncated affinity-improved aptamers for 17β-estradiol determination by AuNPs-based colorimetric aptasensor[J]. Food Chemistry. 2020: 128181.

Citations

1. Lu Z, Cao Y, Zhang D, et al. Discovery of Thieno [2, 3-e] indazole Derivatives as Novel Oral Selective Estrogen Receptor Degraders with Highly Improved Antitumor Effect and Favorable Druggability. Journal of Medicinal Chemistry. 2022 2. Qiao L, Wang H, He J, et al. Truncated affinity-improved aptamers for 17β-estradiol determination by AuNPs-based colorimetric aptasensor. Food Chemistry. 2020: 128181 3. Zhang S, Sun C, Zhao S, et al. Exposure to DEHP or its metabolite MEHP promotes progesterone secretion and inhibits proliferation in mouse placenta or JEG-3 cells. Environmental Pollution. 2020, 257: 113593 4. Liu A, Jin M, Xie L, et al. Loss of miR-29a impairs decidualization of endometrial stromal cells by TET3 mediated demethylation of Col1A1 promoter. Iscience. 2021, 24(9): 103065. 5. Wang Y, Xu G, Wang Z, et al. Psoralidin, a major component of Psoraleae Fructus, induces inflammasome activation and idiosyncratic liver injury. International Immunopharmacology. 2021 Mar;92:107352. doi: 10.1016/j.intimp.2020.107352. Epub 2021 Jan 7. 6. Cui L, Xu F, Jiang Z, et al. Melatonin regulates proliferation and apoptosis of endometrial stromal cells via MT1. Acta Biochimica et Biophysica Sinica. 2021 7. Cui L, Xu F, Xu C, et al.Circadian gene Rev-erbα influenced by sleep conduces to pregnancy by promoting endometrial decidualization via IL-6-PR-C/EBPβ axis.Journal of Biomedical Science.2022, 29(1): 1-14. 8. Qin H, Song Z, Zhao C, et al.miR-363-3p/PTEN is involved in the regulation of lipid metabolism by genistein in HepG2 cells via ERβ.Phytomedicine.2023: 154839.

Related compound libraries

This product is contained In the following compound libraries：

Drug Repurposing Compound Library Anti-Cancer Drug Library Traditional Chinese Medicine Monomer Library EMA Approved Drug Library Human Endogenous Metabolite Library Anti-Cancer Approved Drug Library Anti-Cancer Clinical Compound Library Anti-Ovarian Cancer Compound Library Anti-Inflammatory Traditional Chinese Medicine Compound Library Endocrinology-Hormone Compound Library

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Keywords

Estradiol 50-28-2 Endocrinology/Hormones Metabolism Endogenous Metabolite Estrogen/progestogen Receptor Estrogen Receptor/ERR E2 Estrogen E-2 steroid sex hormone neural differentiation E 2 Inhibitor 17b-Estradiol 17β-Estradiol 17β-Oestradiol 17beta-Estradiol beta-Estradiol 17beta-Oestradiol human endometrial stem cells inhibit β-Estradiol 17b-Oestradiol uterine fibroids b-Estradiol inhibitor

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