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Ifosfamide

Catalog No. T1055 CAS 3778-73-2

Synonyms: NSC109724, Isophosphamide

Ifosfamide (NSC-109724) alkylates and forms DNA crosslinks, thereby preventing DNA strand separation and DNA replication. Ifosfamide is a synthetic analog of the nitrogen mustard cyclophosphamide with antineoplastic activity. This agent is a prodrug that must be activated through hydroxylation by hepatic microsomal enzymes.

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Ifosfamide, CAS 3778-73-2

Pack Size	Availability	Price/USD
100 mg	In stock	$ 41.00
200 mg	In stock	$ 57.00
500 mg	In stock	$ 95.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 100%

Purity: 99.86%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Kinase Assay

cAMP kinase assay: Diced epididymal fat pads from fed Wistar rats (175-225 gm) are obtained after decapitation and incubated at 37 °C for two hours in Krebs-bicarbonate buffer containing 1.27 mM CaCl2. When added, Tolbutamide is present only during the incubation. After incubation fat pads are rinsed and sonicated in cold Krebs-bicarbonate buffer. The aqueous supematants from centrifugation at 50,000 × g for 30 minutes at 4 °C contained 0.75 to 1.25 mg protein per mL and are assayed for cyclic AMP-stimulated protein kinase activity. The assay is performed in 0.2 mL with these additions, 10 μmoles sodium glycerofiosphate pH 7.0, 2 μmoles sodium fluoride, 0.4 μmoles theophylline, 0.1 μmoles ethylene glyool bis (β-aminoethyl ether)-N, N'-tetraaoetic acid, 3 μmoles magnesium chloride, 0.3 mg mixed histone, 2 nmoles (γ- 32P) ATP, 1 nmoles cyclic AMP when indicated, and 0.05 ml of supernatant.

Synonyms	NSC109724, Isophosphamide
Molecular Weight	261.09
Formula	C7H15Cl2N2O2P
CAS No.	3778-73-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 48 mg/mL (183.8 mM)

DMSO: 49 mg/mL (187.7 mM)

Ethanol: 49 mg/mL (187.7 mM)

References and Literature

1. Chang TK, et al. Cancer Res, 1997, 57(10), 1946-1954. 2. Dechant KL, et al. Drugs, 1991, 42(3), 428-467. 3. Chen L, et al. Cancer Res, 1996, 56(6), 1331-1340. 4. Heymann D, et al. Bone, 2005, 37(1), 74-86. 5. Ribeiro RA, et al. J Urol, 2002, 167(5), 2229-2234. 6. Vredenburg G, et al. Activation of the anticancer drugs cyclophosphamide and ifosfamide by cytochrome P450 BM3 mutants. Toxicol Lett. 2015 Jan 5;232(1):182-92. 7. Helal M. Prenatal effects of transplacental exposure to ifosfamide in rats. Biotech Histochem. 2016 Jul;91(5):357-68.

Related compound libraries

This product is contained In the following compound libraries：

Tyrosine Kinase Inhibitor Library Anti-Cancer Approved Drug Library Anti-Cancer Drug Library Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Anti-Cancer Active Compound Library NO PAINS Compound Library Drug-induced Liver Injury (DILI) Compound Library ReFRAME Related Library DNA Damage & Repair Compound Library

Related Products

Related compounds with same targets

N-Nitroso-N-methylurea Chlorambucil RITA Pipobroman Bendamustine hydrochloride Procarbazine hydrochloride Oxaliplatin Cyclophosphamide hydrate

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Ifosfamide 3778-73-2 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA Alkylator/Crosslinker DNA/RNA Synthesis NSC109724 inhibit NSC-109724 Isophosphamide Inhibitor NSC 109724 inhibitor

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