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JIB-04

Catalog No. T1868 CAS 199596-05-9

Synonyms: JIB 04, NSC 693627

JIB-04 (NSC 693627) is a pan-selective Jumonji histone demethylase inhibitor.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

JIB-04, CAS 199596-05-9

Pack Size	Availability	Price/USD
2 mg	In stock	$ 30.00
5 mg	In stock	$ 43.00
10 mg	In stock	$ 68.00
25 mg	In stock	$ 91.00
50 mg	In stock	$ 166.00
100 mg	In stock	$ 288.00
200 mg	In stock	$ 473.00
500 mg	In stock	$ 763.00
1 mL * 10 mM (in DMSO)	In stock	$ 75.00

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Purity: 98.87%

Purity: 98.53%

Purity: 98.44%

Purity: 98.02%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	JIB-04 (NSC 693627) is a pan-selective Jumonji histone demethylase inhibitor.
Targets&IC50	JARID1A:230 nM, JMJD2B:435 nM, JMJD2E:340 nM, JMJD2D:290 nM, JMJD2A:445 nM
In vitro	In the H358 and A549 mouse xenograft models, JIB-04 inhibits the activity of Jumonji domain-containing histone demethylases, thereby suppressing tumor cell growth.
In vivo	In lung and prostate cancer cell lines (IC50=10 nM), JIB-04 downregulates proliferation genes and inhibits cell growth.
Cell Research	For cell viability assays, cells are plated at 1500-3000 cells/well in 96 well plates and treated the next day with increasing doses of compound over 4 days and their viability assessed by standard MTS assays using Promega’s Cell Titer or Cell Titer-Glo reagents according to the manufacturer’s protocols. Absorbance at 490 nm and 650 nm or luminescence is measured by a Spectra Max or a FlouroStar Omega plate reader. Data are normalized to the untreated controls (100% viability). Each cell line is tested in 2-5 independent assays, each containing 4-8 replicates. IC50 values are calculated using DIVISA, a high-throughput software, developed in hous, for storing and analyzing drug sensitivity assays. Dose-response curves are plotted using a non-linear regression model and IC50s are determined from the fitted curves. The average IC50 derived from 2-5 independent assays, each containing 4-8 replicates is reported. (Only for Reference)

Synonyms	JIB 04, NSC 693627
Molecular Weight	308.76
Formula	C17H13ClN4
CAS No.	199596-05-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 30.9 mg/mL (100 mM)

References and Literature

1. Wang L, et al. Nat Commun. 2013, 4, 2035.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Aging Compound Library Bioactive Compound Library NO PAINS Compound Library Methylation Compound Library Apoptosis Compound Library Epigenetics Compound Library Reprogramming Compound Library Stem Cell Differentiation Compound Library Bioactive Compounds Library Max

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

JIB-04 199596-05-9 Apoptosis Chromatin/Epigenetic Histone Demethylase Histone Methyltransferase Inhibitor JIB 04 NSC693627 NSC-693627 inhibit NSC 693627 JIB04 inhibitor

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