Powder: -20°C for 3 years | In solvent: -80°C for 1 year
JSH-23, an NF-κB inhibitor(IC50= 7.1 μM), suppresses the transcriptional activity.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 36.00 | |
2 mg | In stock | $ 52.00 | |
5 mg | In stock | $ 85.00 | |
10 mg | In stock | $ 122.00 | |
25 mg | In stock | $ 197.00 | |
50 mg | In stock | $ 297.00 | |
100 mg | In stock | $ 453.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 94.00 |
Description | JSH-23, an NF-κB inhibitor(IC50= 7.1 μM), suppresses the transcriptional activity. |
Targets&IC50 | NF-κB:7.1 μM |
In vitro | Within diabetic rat models, JSH-23 (3 mg/kg) effectively reduces neuroinflammation and enhances antioxidant defenses, significantly reversing neural conduction impairments and ultimately ameliorating insufficient neurovascular perfusion. |
In vivo | In RAW 264.7 cells, JSH-23 at concentrations below 100 μM did not exhibit significant cytotoxic effects and was capable of inhibiting lipopolysaccharide-(LPS)-induced apoptotic chromatin condensation. Furthermore, in primary cerebellar neurons of mice, JSH-23 inhibited the LPS-induced nuclear translocation of NF-κB p65 without affecting the degradation of IκBα. |
Kinase Assay | Measurement of NF-κB transcriptional activity: Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm. |
Cell Research | Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.(Only for Reference) |
Molecular Weight | 240.34 |
Formula | C16H20N2 |
CAS No. | 749886-87-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 45 mg/mL (187.2 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 16 mg/mL (66.6 mM)
You can also refer to dose conversion for different animals. More
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JSH-23 749886-87-1 NF-Κb NF-κB Nuclear factor-κB Nuclear factor-kappaB Inhibitor JSH 23 JSH23 inhibit inhibitor