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MI-3

Catalog No. T2643 CAS 1271738-59-0

Synonyms: Menin-MLL Inhibitor, Menin-MLL inhibitor 3, MI 3

MI-3 (Menin-MLL Inhibitor) (Menin-MLL Inhibitor) is an effective inhibitor of Menin-MLL interaction (IC50: 648 nM).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

MI-3, CAS 1271738-59-0

Pack Size	Availability	Price/USD
1 mg	In stock	$ 56.00
5 mg	In stock	$ 128.00
10 mg	In stock	$ 197.00
25 mg	In stock	$ 338.00
50 mg	In stock	$ 491.00
1 mL * 10 mM (in DMSO)	In stock	$ 140.00

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Purity: 98.97%

Purity: 98.66%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	MI-3 (Menin-MLL Inhibitor) (Menin-MLL Inhibitor) is an effective inhibitor of Menin-MLL interaction (IC50: 648 nM).
Targets&IC50	Menin-MLL:648 nM
Kinase Assay	High Throughput Screening: FITC-MBM1 at 15 nM and menin at 150 nM in the FP buffer are mixed and incubated for 1h in the dark at room temperature. For point screening, the 0.2 μL of each compound (20 μM final concentration, 1% DMSO) is added to 20 μL of the aliquot of the protein-peptide mixture and incubated on 384-well plates in the dark at room temperature for 1h. In confirmation screening, the serial dilution plates with compounds in DMSO are prepared and used to titrate the menin-FITC-MBM1 complex. Change in fluorescence polarization is monitored at 525 nm after excitations at 495 nm using the PHERAstar microplate reader (BMG) and applied to determine IC50 values with the Origin 7.0 program.
Cell Research	The MLL-AF9 and E2A-HLF transduced murine BMC are plated in 12-well plates at the concentration of 5×103 cells/mL with 1 mL methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained with 100 μL iodonitrotetrazolium chloride at final concentration of 1 mg/mL, incubated at 37°C for 30 min and counted. To replate for the 2nd round, colonies are counted at day 6 without staining and cells were washed out by 1×PBS buffer and resuspended in IMDM medium containing 15% FBS, 1% penicillin/streptomycin and IL-3. 5×103 cells are plated in 12-well plates with 1ml methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained and counted. (Only for Reference)

Synonyms	Menin-MLL Inhibitor, Menin-MLL inhibitor 3, MI 3
Molecular Weight	375.55
Formula	C18H25N5S2
CAS No.	1271738-59-0

Storage

store at low temperature,keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 16 mg/mL (42.6 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 16 mg/mL (42.6 mM)

References and Literature

1. Grembecka J, et al. Nat Chem Biol. 2012, 8(3), 277-284.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library PPI Inhibitor Library Histone Modification Compound Library NO PAINS Compound Library Chromatin Modification Compound Library Bioactive Compounds Library Max Apoptosis Compound Library Bioactive Compound Library Epigenetics Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

MI-3 1271738-59-0 Apoptosis Chromatin/Epigenetic Histone Methyltransferase Epigenetic Reader Domain Menin-MLL Inhibitor MI3 Menin-MLL inhibitor 3 inhibit Inhibitor MI 3 inhibitor

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