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NSC 185058

Catalog No. T5211 CAS 39122-38-8

Synonyms: NSC185058

NSC 185058 is an inhibitor of ATG4B, a major cysteine protease. It also can attenuate the autophagic activity.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

NSC 185058, CAS 39122-38-8

Pack Size	Availability	Price/USD
1 mg	In stock	$ 88.00
5 mg	In stock	$ 189.00
10 mg	In stock	$ 297.00
25 mg	In stock	$ 516.00
50 mg	In stock	$ 736.00
100 mg	In stock	$ 987.00

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Purity: 99.83%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	NSC 185058 is an inhibitor of ATG4B, a major cysteine protease. It also can attenuate the autophagic activity.
In vitro	NSC185058 effectively suppressed starvation-induced autophagy in both cell types. GFP-LC3B labeled AVs were absent in starved MDA-MB468 cells when treated with NSC185058. The inhibitory effects of both NSC185058 on starvation-induced protein turnover were comparable to 3MA.
In vivo	Mice were injected at 2 24 h intervals with vehicle or NSC185058, autophagy induced by 20 h fasting. Fasting resulted in an increase of GFP-LC3B AVs compared with ad libitum fed mice. NSC185058 significantly decreased the appearance of GFP-LC3B AVs compared with fasted mice.
Cell Research	Saos-2 transiently expressing FYVE-RFP was incubated in DMEM medium supplemented with amino acids and serum (Fed) or Krebs-Heinseleit medium lacking amino acids and serum (Starved). The starved cells were incubated in the presence and absence of the NSC185058 or NSC377071 for 4 h at which time the cells were examined by fluorescence microscopy. The numbers of red "dots" per cell was quantified using ImageJ software. Taking into account basal responses, those cells with 3 or more puncta were defined as PtdIns3K active.
Animal Research	Immunodeficient nu/nu nude female mice were injected subcutaneously with 6 × 10^6 Saos-2 (GFP-LC3B), shCon-Saos-2(GFP-LC3B) or shATG4B-Saos-2(GFP-LC3B) cells. Palpable Saos-2 (GFP-LC3B) tumors developed in 7 to 10 d, at which time, the mice were divided into 2 groups and injected IP on Monday, Wednesday, and Friday with either peanut oil vehicle or NSC185058 (100 mg/kg body weight) dissolved in peanut oil. Over time, the tumor dimensions were measured using calipers and volumes calculated using the formula: (W2 × L)/2.

Synonyms	NSC185058
Molecular Weight	215.27
Formula	C11H9N3S
CAS No.	39122-38-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 30 mg/mL (139.36 mM)

Ethanol: 2.5 mg/mL

References and Literature

1. Akin D, et al. A novel ATG4B antagonist inhibits autophagy and has a negative impact on osteosarcoma tumors. Autophagy. 2014;10(11):2021-35.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library NO PAINS Compound Library Protease Inhibitor Library Bioactive Compound Library Bioactive Compounds Library Max Autophagy Compound Library

Related Products

Related compounds with same targets

Dazcapistat 2-Aminoethanethiol Aurantiamide acetate Z-FA-FMK Aloxistatin KGP94 MK-0674 JTE-607

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

NSC 185058 39122-38-8 Autophagy Proteases/Proteasome Cysteine Protease Inhibitor NSC185058 Atg4 Autophagy-related 4 NSC-185058 inhibit inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.