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Pitstop 2

Catalog No. T26357Cas No. 1419320-73-2

Pitstop2 is an amphipathic protein-bound inhibitor of the clathrin terminal domain (IC50=12 μM) with selectivity. Pitstop2 severely interferes with receptor-mediated endocytosis, HIV entry and synaptic vesicle cycling. Pitstop2 has antitumor activity.

Pitstop 2

Pitstop 2

Purity: 99.53%
Catalog No. T26357Cas No. 1419320-73-2
Pitstop2 is an amphipathic protein-bound inhibitor of the clathrin terminal domain (IC50=12 μM) with selectivity. Pitstop2 severely interferes with receptor-mediated endocytosis, HIV entry and synaptic vesicle cycling. Pitstop2 has antitumor activity.
Pack SizePriceAvailabilityQuantity
1 mg$34In Stock
2 mg$48In Stock
5 mg$80In Stock
10 mg$139In Stock
25 mg$289In Stock
50 mg$455In Stock
100 mg$672In Stock
1 mL x 10 mM (in DMSO)$100In Stock
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Purity:99.53%
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Product Introduction

Bioactivity
Description
Pitstop2 is an amphipathic protein-bound inhibitor of the clathrin terminal domain (IC50=12 μM) with selectivity. Pitstop2 severely interferes with receptor-mediated endocytosis, HIV entry and synaptic vesicle cycling. Pitstop2 has antitumor activity.
In vitro
METHODS: HeLa cells were pre-incubated with Pitstop2 (10-30 µM) for 15 min, followed by incubation with Alexa568-Tf for 15 min, and transferrin (Tf) uptake was detected by live cell microscopy.
RESULTS: HeLa cells efficiently internalized Tf into perinuclear circulating endosomes when exposed to Alexa56-Tf. Preincubation of HeLa cells with Pitstop2 resulted in a dose-dependent inhibition of Tf uptake with IC50 values of 12-15 µM, which was very similar to that measured by in vitro blockade of the function of lattice protein TD. [1]
METHODS: Asynchronously growing HeLa cells were treated with Pitstop2 (0.001-100 µM) for 6 h and assayed for mitotic index.
RESULTS: Acute treatment of HeLa cells with Pitstop2 for 6 h resulted in a significant increase in mitotic index, suggesting that Pitstop2 activates the SAC. The effect of Pitstop2 on mitotic index was concentration-dependent, and significant at concentrations as low as 0.01 µM. [2]
Chemical Properties
Molecular Weight473.36
FormulaC20H13BrN2O3S2
Cas No.1419320-73-2
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 6.88 mg/mL (14.52 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.1126 mL10.5628 mL21.1256 mL105.6279 mL
5 mM0.4225 mL2.1126 mL4.2251 mL21.1256 mL
10 mM0.2113 mL1.0563 mL2.1126 mL10.5628 mL

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