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Puromycin dihydrochloride

Catalog No. T2219 CAS 58-58-2

Synonyms: CL13900 dihydrochloride, Puromycin 2HCl

Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity.

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Puromycin dihydrochloride Chemical Structure

Puromycin dihydrochloride, CAS 58-58-2

Pack Size	Availability	Price/USD
10 mg	In stock	$ 39.00
25 mg	In stock	$ 70.00
50 mg	In stock	$ 122.00
100 mg	In stock	$ 197.00
1 mL * 10 mM (in DMSO)	In stock	$ 43.00

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Purity: 99.86%

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Purity: 99.59%

Purity: 99.31%

Purity: 98.97%

Purity: 98.65%

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Description	Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity.
In vitro	METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT. RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively.[1] METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection. RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. [2] METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL). RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells. [3]
In vivo	Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems.

Synonyms	CL13900 dihydrochloride, Puromycin 2HCl
Molecular Weight	544.44
Formula	C22H31Cl2N7O5
CAS No.	58-58-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 92 mg/mL (169 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 54.4 mg/mL(100 mM)

References and Literature

1. Chan C, et al. Incompatibility of chemical protein synthesis inhibitors with accurate measurement of extended protein degradation rates. Pharmacol Res Perspect. 2017 Oct;5(5):e00359. 2. Han W, et al. Roles of eIF3m in the tumorigenesis of triple negative breast cancer. Cancer Cell Int. 2020 Apr 29;20:141. 3. Wang YN, et al. Targeting the cholesterol-RORα/γ axis inhibits colorectal cancer progression through degrading c-myc. Oncogene. 2022 Dec;41(49):5266-5278. 4. Han W, Zhang C, Shi C, et al. Roles of eIF3m in the tumorigenesis of triple negative breast cancer[J]. Cancer Cell International. 2020, 20(1): 1-16. 5. Gao C, Wang H, Wang T, et al. Platelet CLEC‐2 regulates neuroinflammation and restores blood brain barrier integrity in a mouse model of traumatic brain injury[J]. Journal of Neurochemistry. 2020: e14983.

Citations

1. Wang Y N, Ruan D Y, Wang Z X, et al. Targeting the cholesterol-RORα/γ axis inhibits colorectal cancer progression through degrading c-myc. Oncogene. 2022: 1-13. 2. Gao C, Wang H, Wang T, et al. Platelet CLEC‐2 regulates neuroinflammation and restores blood brain barrier integrity in a mouse model of traumatic brain injury. Journal of Neurochemistry. 2020: e14983 3. Han W, Zhang C, Shi C, et al. Roles of eIF3m in the tumorigenesis of triple negative breast cancer. Cancer Cell International. 2020, 20(1): 1-16 4. Han W, Cao F, Gao X J, et al. ZIC1 acts a tumor suppressor in breast cancer by targeting survivin. International Journal of Oncology. 2018 Sep;53(3):937-948

Related compound libraries

This product is contained In the following compound libraries：

Antiparasitic Natural Product Library Anti-Parasitic Compound Library Anti-Cancer Active Compound Library Inhibitor Library Microbial Natural Product Library Natural Product Library for HTS Cell Cycle Compound Library Covalent Inhibitor Library Bioactive Compound Library Anti-Bacterial Compound Library

Related Products

Related compounds with same targets

Methacycline hydrochloride Demeclocycline hydrochloride Gentamicin sulfate Doxycycline Amikacin sulfate Erythromycin ethylsuccinate Clindamycin phosphate Azithromycin hydrate

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Keywords

Puromycin dihydrochloride 58-58-2 Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology Antibacterial DNA/RNA Synthesis ribosome Antibiotic CL13900 dihydrochloride Puromycin 2HCl Puromycin Bacterial Inhibitor Puromycin Dihydrochloride inhibit CL 13900 Dihydrochloride CL-13900 CL-13900 Dihydrochloride CL 13900 CL13900 Dihydrochloride CL13900 inhibitor

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