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Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 mg | $39 | In Stock | |
25 mg | $70 | In Stock | |
50 mg | $122 | In Stock | |
100 mg | $197 | In Stock | |
1 mL x 10 mM (in H2O) | $43 | In Stock |
Description | Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity. |
In vitro | METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT. RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively.[1] METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection. RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. [2] METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL). RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells. [3] |
In vivo | Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems. |
Alias | Puromycin 2HCl, CL13900 dihydrochloride |
Molecular Weight | 544.44 |
Formula | C22H31Cl2N7O5 |
Cas No. | 58-58-2 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
Solubility Information | H2O: 92 mg/mL (169 mM) Ethanol: < 1 mg/mL (insoluble or slightly soluble) DMSO: 60 mg/mL (110.2 mM) | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO/H2O
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