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Puromycin dihydrochloride

Puromycin dihydrochloride
Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity.
Catalog No. T2219Cas No. 58-58-2
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Purity:99.86%
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Puromycin dihydrochloride

Purity: 99.86%
Catalog No. T2219Alias Puromycin 2HCl, CL13900 dihydrochlorideCas No. 58-58-2

Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
10 mg$39In Stock
25 mg$70In Stock
50 mg$122In Stock
100 mg$197In Stock
1 mL x 10 mM (in H2O)$43In Stock
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Product Introduction

Bioactivity
Description
Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity.
In vitro
METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT.
RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively.[1]
METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection.
RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. [2]
METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL).
RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells. [3]
In vivo
Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems.
AliasPuromycin 2HCl, CL13900 dihydrochloride
Chemical Properties
Molecular Weight544.44
FormulaC22H31Cl2N7O5
Cas No.58-58-2
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
H2O: 92 mg/mL (169 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 60 mg/mL (110.2 mM)
Solution Preparation Table
DMSO/H2O
1mg5mg10mg50mg
1 mM1.8367 mL9.1837 mL18.3675 mL91.8375 mL
5 mM0.3673 mL1.8367 mL3.6735 mL18.3675 mL
10 mM0.1837 mL0.9184 mL1.8367 mL9.1837 mL
20 mM0.0918 mL0.4592 mL0.9184 mL4.5919 mL
50 mM0.0367 mL0.1837 mL0.3673 mL1.8367 mL
100 mM0.0184 mL0.0918 mL0.1837 mL0.9184 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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