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Pyrrolidinedithiocarbamate ammonium

Catalog No. T3147 CAS 5108-96-3

Synonyms: PDTC, Pyrrolidinedithiocarbamate, APDC, 1-Pyrrolidinedithiocarboxylic acid ammonium salt, Ammonium pyrrolidinedithiocarbamate

Pyrrolidinedithiocarbamate ammonium (1-Pyrrolidinedithiocarboxylic acid ammonium salt), a selective NF-κB inhibitor, inhibits translation of nitric oxide synthase mRNA to prevent induction.

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Pyrrolidinedithiocarbamate ammonium Chemical Structure

Pyrrolidinedithiocarbamate ammonium, CAS 5108-96-3

Pack Size	Availability	Price/USD
100 mg	In stock	$ 41.00
200 mg	In stock	$ 58.00
500 mg	In stock	$ 96.00
1 mL * 10 mM (in DMSO)	In stock	$ 39.00

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Pyrrolidinedithiocarbamate ammonium (1-Pyrrolidinedithiocarboxylic acid ammonium salt), a selective NF-κB inhibitor, inhibits translation of nitric oxide synthase mRNA to prevent induction.
In vitro	Pretreatment of cells with PDTC (3-1000 mM) dose-dependently attenuate IL-8 production. Furthermore, PDTC (100 mM) suppresses the accumulation of IL-8 mRNA. PDTC inhibites the activation of NF-kB, because PDTC suppresses both NF-kB DNA binding and NF-kB-dependent transcriptional activity. Taken together, our data demonstrate that NF-kB inhibition with PDTC decrease IL-8 production by intestinal epithelial cells[1].
In vivo	The DSS+PDTC-treated groupⅡ exhibited suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels are significantly lower in DSS+PDTC-treated groupⅡ. These findings suggest that suppression of NF-κB activity by PDTC can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1β and TNF-α), resulting in significant alleviation of colitis. PDTC is useful for the treatment of ulcerative colitis[2].
Kinase Assay	All binding studies are performed in an HTRF assay buffer consisting of dPBS supplemented with 0.1% (with v) bovine serum albumin and 0.05% (v/v) Tween-20. For the PD-l-Ig/PD-Ll-His binding assay, inhibitors are pre-incubated with PD-Ll-His (10 nM final) for 15 m in 4 μL of assay buffer, followed by addition of PD-l-Ig (20 nM final) in 1 μL of assay buffer and further incubation for 15 m. PD-L1 from either human, cyno, or mouse are used. HTRF detection is achieved using europium crypate-labeled anti- Ig (1 nM final) and allophycocyanin (APC) labeled anti-His (20 nM final). Antibodies are diluted in HTRF detection buffer and 5 μL is dispensed on top of binding reaction. The reaction mixture is allowed to equilibrate for 30 minutes and signal (665 nm/620 nm ratio) is obtained using an En Vision fluorometer. Additional binding assays are established between PD-1-Ig/PD-L2-His (20, 5 nM, respectively), CD80-His/PD-Ll-Ig (100, 10 nM, respectively) and CD80-His/CTLA4-Ig (10, 5 nM, respectively).
Cell Research	The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy's 5A medium supplemented with 10% fetal bovine serum. To study the effect of PDTC on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1b for 18 h. Various concentrations (3-1000 mM) of PDTC or its vehicle (culture medium) are added to the cells 30 min prior to IL-1b stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay, as described previously employing the multiple antibody sandwich principle that specifically detects human IL-8[1].

Synonyms	PDTC, Pyrrolidinedithiocarbamate, APDC, 1-Pyrrolidinedithiocarboxylic acid ammonium salt, Ammonium pyrrolidinedithiocarbamate
Molecular Weight	164.29
Formula	C5H12N2S2
CAS No.	5108-96-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 16.4 mg/mL(100 mM)

References and Literature

1. Qin, J., Cao, Z., Li, X., Kang, X., Xue, Y., & Li, Y. et al. (2014). Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury. Pharmaceutical Biology, 52(11), 1460-1466. doi: 10.3109/13880209.2014.898075 2. Gao Y, Elamin E, Zhou R, et al. FKBP51 promotes migration and invasion of papillary thyroid carcinoma through NF‑κB‑dependent epithelial‑to‑mesenchymal transition[J]. Oncology letters. 2018 Dec;16(6):7020-7028. 3. Lu S, Luo Y, Sun G B, et al. Ginsenoside compound K attenuates ox-LDL-mediated macrophage inflammation and foam cell formation via autophagy induction and modulating NF-κB, p38, and JNK MAPK signaling[J]. Frontiers in Pharmacology. 2020, 11. 4. Zhao L, Xia M, Wang K, et al. A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon β1 and ISG Expression[J]. Frontiers in Microbiology. 2020, 11: 260. 5. Shi F1, Deng Z1, Zhou Z2, Jiang CY1, Zhao RZ1, Sun F1,3, Cui D1,3, Bei XY1,3, Yang BY1, Sun Q2, Wang XJ1,3, Wu Q2, Xia SJ1,3, Han BM1,3. QKI‐6 inhibits bladder cancer malignant behaviours through down‐regulating E2F3 and NF‐κB signalling. Journal of cellular and molecular medicine. 6. Li Y, Niu M, Zhao A, et al. CXCL12 is involved in α-synuclein-triggered neuroinflammation of Parkinson’s disease[J]. Journal of Neuroinflammation. 2019, 16(1): 1-14.

Citations

1. Zhu T, Chen X, Qiu H, et al. Aspirin Alleviates Particulate Matter Induced Asymptomatic Orchitis of Mice via Suppression of cGAS-STING Signaling. Frontiers in immunology. 2021, 12. 2. Guo T, Liu C, Yang C, et al. Immunoproteasome subunit PSMB8 regulates microglia-mediated neuroinflammation upon manganese exposure by PERK signaling. Food and Chemical Toxicology. 2022: 112951. 3. Li Y, Niu M, Zhao A, et al. CXCL12 is involved in α-synuclein-triggered neuroinflammation of Parkinson’s disease. Journal of Neuroinflammation. 2019, 16(1): 1-14 4. Shi F1, Deng Z1, Zhou Z2, Jiang CY1, Zhao RZ1, Sun F1,3, Cui D1,3, Bei XY1,3, Yang BY1, Sun Q2, Wang XJ1,3, Wu Q2, Xia SJ1,3, Han BM1,3. QKI‐6 inhibits bladder cancer malignant behaviours through down‐regulating E2F3 and NF‐κB signalling. Journal of Cellular and Molecular Medicine. 2019, 23(10): 6578-6594 5. Zhao L, Xia M, Wang K, et al. A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon β1 and ISG Expression. Frontiers in Microbiology. 2020, 11: 260 6. Lu S, Luo Y, Sun G B, et al. Ginsenoside compound K attenuates ox-LDL-mediated macrophage inflammation and foam cell formation via autophagy induction and modulating NF-κB, p38, and JNK MAPK signaling. Frontiers in Pharmacology. 2020 Sep 15;11:567238 7. Wang Y, Gao W. Effects of TNF-α on autophagy of rheumatoid arthritis fibroblast-like synoviocytes and regulation of the NF-κB signaling pathway. Immunobiology. 2021, 226(2): 152059. 8. Gao Y, Elamin E, Zhou R, et al. FKBP51 promotes migration and invasion of papillary thyroid carcinoma through NF‑κB‑dependent epithelial‑to‑mesenchymal transition. Oncology letters. 2018 Dec;16(6):7020-7028. 9. Tian X, Liu F, Wang Z, et al.Modified Biejia Jianwan decoction restrains PD-L1-mediated immune evasion through the HIF-1α/STAT3/NF-κB signaling pathway.Journal of Ethnopharmacology.2023: 117577.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Inhibitor Library Anti-Cancer Drug Library Drug Repurposing Compound Library Anti-Cancer Active Compound Library Pyroptosis Compound Library Anti-Aging Compound Library Anti-Lung Cancer Compound Library HIF-1 Signaling Pathway Compound Library Anti-Breast Cancer Compound Library

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Keywords

Pyrrolidinedithiocarbamate ammonium 5108-96-3 NF-Κb NF-κB TNF-α Inhibitor 1-Pyrrolidinedithiocarboxylic acid ammonium PDTC Nuclear factor-κB inhibit Pyrrolidinedithiocarbamate APDC 1-Pyrrolidinedithiocarboxylic acid ammonium salt ulcerative Ammonium pyrrolidinedithiocarbamate inflammation IL-1β colitis Nuclear factor-kappaB inhibitor

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