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Reversine

Catalog No. T1825 CAS 656820-32-5

Purity 99.75% Datasheet

Reversine, a small synthetic purine analogue (2, 6-disubstituted purine), is a potent inhibitior of Aurora A/B/C(IC50s=150-500 nM).

Reversine, CAS 656820-32-5

Pack Size	Availability	Price/USD
2 mg	In stock	50.00
5 mg	In stock	72.00
10 mg	In stock	90.00
25 mg	In stock	186.00
50 mg	In stock	262.00
100 mg	In stock	460.00
200 mg	In stock	828.00
1 mL * 10 mM (in DMSO)	In stock	72.00

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Purity 98.00%

HNMR COA Datasheet MSDS

Purity 99.75%

HNMR HPLC COA Datasheet MSDS

Purity 98.00%

HNMR COA Datasheet MSDS

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Reversine, a small synthetic purine analogue (2, 6-disubstituted purine), is a potent inhibitior of Aurora A/B/C(IC50s=150-500 nM).
Targets&IC50	A3 :ic50 0.66μM(Ki), Aurora A :ic50 12nM, Aurora B :ic50 13nM, Aurora C :ic50 20nM,
Kinase Assay	Radioligand Binding Assays: Each tube in the A3 AR competitive binding assay contains 100 μL of membrane suspension (20 μg of protein), 50 μL of [125I]4-amino-3-iodobenzyl)adenosine-5′-N-methyluronamide (0.5 nM), and 50 μL of increasing concentrations of the test ligands in Tris-HCl buffer (50 mM, pH 7.4) containing 10 mM MgCl2 and 1 mM EDTA. Nonspecific binding is determined using 10 mM 5′-N-ethylcarboxamidoadenosine in the buffer. The mixtures are incubated at 25°C for 60 min. Binding reactions are terminated by filtration through Whatman GF/B filters under reduced pressure using a MT-24 cell harvester. Filters are washed three times with 9 mL of ice-cold buffer. Radioactivity is determined using a Beckman γ-counter, and the percent inhibition is calculated.
Cell Research	Cell viability of different tumor cell lines is assessed using ATPlite 1step. Briefly, 2 × 104 cells for each well are plated in a 96-well plate in presence of crescent quantity of reversine. After 72 h, the plates are recovered and 100 μL ATPlite solution is added to each well. The plates are shaken for 2 min at 700 rpm and luminescence is measured using EnVision Multilabel plate reader. Each sample is analyzed in triplicate.(Only for Reference) Cell lines: HL-60, A375, HeLa, HCT-116, T47D, and MCF-7 cell lines
Animal Research	Animal Model: Mice bearing U14 tumors

Molecular Weight	393.49
Formula	C21H27N7O
CAS No.	656820-32-5

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 5 mg/mL (12.71 mM), warmed

Ethanol: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature

1. Chen S, et al. J Am Chem Soc. 2004, 126(2), 410-411. 2. Perreira M, et al. J Med Chem. 2005, 48(15), 4910-4918. 3. D'Alise AM, et al. Mol Cancer Ther. 2008, 7(5), 1140-1149. 4. Qin HX, et al. Cytotechnology. 2013, 65(4), 643-653.

Related compound libraries

This product is contained In the following compound libraries：

Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Clinical Compound Library Stem cell Differentiation Compound Library Autophagy Compound Library Neuronal Signaling Compound Library Epigenetics Compound Library DNA Damage & Repair Compound Library Kinase Inhibitor Library GPCR Compound Library Anti-cancer Clinical Compound Library Cell cycle related Compound Library

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