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SKL2001

Catalog No. T6989 CAS 909089-13-0

SKL2001, an agonist of the Wnt/β-catenin pathway, can disrupt the Axin/β-catenin interaction.

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SKL2001, CAS 909089-13-0

Pack Size	Availability	Price/USD
5 mg	In stock	$ 34.00
10 mg	In stock	$ 55.00
25 mg	In stock	$ 109.00
50 mg	In stock	$ 195.00
100 mg	In stock	$ 357.00
200 mg	In stock	$ 537.00
500 mg	In stock	$ 839.00
1 mL * 10 mM (in DMSO)	In stock	$ 61.00

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Purity: 99.5%

Purity: 97.90%

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Description	SKL2001, an agonist of the Wnt/β-catenin pathway, can disrupt the Axin/β-catenin interaction.
In vitro	SKL2001 upregulated β-catenin responsive transcription by increasing the intracellular β-catenin protein level and inhibited the phosphorylation of β-catenin at residues Ser33/37/Thr41 and Ser45, which would mark it for proteasomal degradation, without affecting CK1 and GSK-3β enzyme activities. SKL2001 disrupted the Axin/β-catenin interaction, which is a critical step for CK1- and GSK-3β-mediated phosphorylation of β-catenin at Ser33/37/Thr41 and Ser45. The treatment of mesenchymal stem cells with SKL2001 promoted osteoblastogenesis and suppressed adipocyte differentiation, both of which were accompanied by the activation of Wnt/β-catenin pathway. SKL2001 did not affect either NF-κB or p53 reporter activity and inhibits β-catenin phosphorylation without affecting GSK-3β activity[1].
Cell Research	The HEK293 reporter and control cell lines were established. The HEK293 reporter cells were inoculated into 384-well plates at 10 000 cells per well and grown for 24 h. Next, each compound in the chemical library (～270 000) was added to at a final concentration of 20 μM. After 15 h, the plates were assayed for firefly luciferase activity.(Only for Reference)

Molecular Weight	286.29
Formula	C14H14N4O3
CAS No.	909089-13-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 53 mg/mL (185.1 mM)

Ethanol: 53 mg/mL (185.1 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Gwak J, et al. Cell Res. 2012, 22(1):237-47. 2. Lu F, Sun X, Xu X, et al. SILAC-based proteomic profiling of the suppression of TGF-β1-induced lung fibroblast-to-myofibroblast differentiation by trehalose[J]. Toxicology and Applied Pharmacology. 2020, 391: 114916. 3. Lu F, Sun X, Xu X, et al. SILAC-based proteomic profiling of the suppression of TGF-β1-induced lung fibroblast-to-myofibroblast differentiation by trehalose[J]. Toxicology and Applied Pharmacology. 2020: 114916.

Citations

1. Liu X, Xie P, Hao N, et al. HIF-1–regulated expression of calreticulin promotes breast tumorigenesis and progression through Wnt/β-catenin pathway activation. Proceedings of the National Academy of Sciences. 2021, 118(44) 2. Cheng X, Qin L, Deng L, et al. SNX-2112 Induces Apoptosis and Inhibits Proliferation, Invasion, and Migration of Non-Small Cell Lung Cancer by Downregulating Epithelial-Mesenchymal Transition via the Wnt/β-Catenin Signaling Pathway. Journal of Cancer. 2021, 12(19): 5825-5837. 3. Lu F, Sun X, Xu X, et al. SILAC-based proteomic profiling of the suppression of TGF-β1-induced lung fibroblast-to-myofibroblast differentiation by trehalose. Toxicology and Applied Pharmacology. 2020: 114916 4. Yan Z, Ruan B, Wang S, et al.RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway.Archives of Medical Research.2023: 102853. 5. Bi G, Liang J, Bian Y, et al.Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer.Nature Communications.2024, 15(1): 2461.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Anti-Fibrosis Compound Library Cancer Cell Differentiation Compound Library Neuronal Differentiation Compound Library Neural Regeneration Compound Library Anti-Colorectal Cancer Compound Library Stem Cell Differentiation Compound Library Bioactive Compounds Library Max NO PAINS Compound Library CNS-Penetrant Compound Library

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β-catenin/CBP-IN-1 β-catenin modulator IIa-661 Wnt pathway activator 1 IWP-2 MN-64 C-82 Tankyrase-IN-2 SSTC3

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Keywords

SKL2001 909089-13-0 Cytoskeletal Signaling Stem Cells Wnt/beta-catenin Inhibitor Wnt Beta catenin β-catenin SKL-2001 inhibit SKL 2001 inhibitor

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