Angiogenesis FGFR TAK-779


Catalog No. T7499   CAS 229005-80-5
Synonyms: Takeda 779

TAK-779 is an antagonist of chemokine receptor 5 (CCR5), CCR2b, and CXC chemokine receptor 3 (CXCR3).

TAK-779, CAS 229005-80-5
Pack Size Availability Price/USD Quantity
2 mg In stock 75.00
5 mg In stock 107.00
10 mg In stock 195.00
25 mg In stock 351.00
50 mg In stock 527.00
1 mL * 10 mM (in DMSO) In stock 203.00
Bulk Inquiry
Select Batch  
Purity 98.00%
Purity 98.43%
Biological Description
Chemical Properties
Storage & Solubility Information
Preparing Solutions
Description TAK-779 is an antagonist of chemokine receptor 5 (CCR5), CCR2b, and CXC chemokine receptor 3 (CXCR3).
Targets&IC50 Abl :ic50 14nM,   Abl (T315I) :ic50 4nM,   Aurora A :ic50 48nM,   FGFR1 :ic50 36nM,   FLT3 :ic50 6.6nM,   FLT3 (D835Y) :ic50 1nM,   JAK2 :ic50 150nM,   Kit :ic50 300nM,   Src :ic50 400nM,   MIP-1α-CCR5 :ic50 1 nM (in CHO/CCR5 cells),   MIP-1β-CCR5 :ic50 1 nM (in CHO/CCR5 cells),   RANTES-CCR5 :ic50 1.4 nM (in CHO/CCR5 cells),   MCP-1-CCR2b :ic50 27 nM (in CHO/CCR5 cells),   R5 HIV-1 (Ba-L) :ic50 3.7 nM (EC50, in PBMCs),   R5 HIV-1 (KK) :ic50 1.6 nM (EC50, in PBMCs),   CCR2;CXCR,  
In vivo TAK-779 increases intestinal allograft survival in a rat model of small intestine transplantation when administered at a dose of 10 mg/kg per day[2].
Kinase Assay FLT3 phosphorylation: Leukemia cells are washed in phosphate-buffered saline (PBS), then lysed by resuspending the cells in lysis buffer (20 mM Tris pH 7.4, 100 mM NaCl, 1% Igepal, 1 mM EDTA, 2 mM NaVO4, plus Complete protease inhibitor KW-2449 for 30 minutes while rocking. The extract is clarified by centrifugation at 1.6 × 104 g and the supernatant is assayed for protein (Bio-Rad). A 50-μg aliquot is removed as a whole-cell lysate for analysis of STAT5, and the remainder is used for immunoprecipitation with anti-FLT3. Anti-FLT3 antibody is added to the extract for overnight incubation, then protein A sepharose is added for 2 additional hours. Separate sodium dodecyl sulfate–polyacrylamide electrophoresis (SDS-PAGE) gels for whole-cell lysate and immunoprecipates are run in parallel. After transfer to Immobilon membranes, immunoblotting is performed with antiphosphotyrosine antibody (4G10) to detect phosphorylated FLT3 or, for the whole-cell lysate gels, with a rat monoclonal antibody against phosphorylated STAT5 (residue Y694) then stripped and reprobed with anti-FLT3 antibody to measure total FLT3. Proteins are visualized using chemiluminescence, exposed on Kodak BioMax XAR film, developed, and scanned using a Bio-Rad GS800 densitometer. The concentration of KW-2449 for which the phosphorylation of FLT3 or STAT5 is inhibited to 50% of its baseline (IC50) is determined using linear regression analysis of the dose response curves. For direct analysis of FLT3 and STAT5 in circulating blasts, peripheral blood is collected in heparinized tubes and promptly chilled on ice. Samples are centrifuged for 10 minutes at 900 g, at 4 °C. The plasma is removed and stored frozen at −80 °C. The buffy coat is carefully transferred to ice-cold PBS, layered onto chilled Ficoll-Hypaque, and centrifuged for 5 minutes at 600 g, at 4 °C. All subsequent steps are carried out at 4 °C. Mononuclear cells are collected and washed rapidly once in red blood cell lysis buffer (0.155 M NH4Cl, 0.01 M KHCO3, 0.1 mM EDTA), then washed once in PBS. Cells are then lysed as described for FLT3 and STAT5 analysis.
Cell Research
Cell viability is determined by the sodium 3′-[1-(phenylaminocarbonyl)-3, 4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate assay after incubation with or without KW-2449 for 72 hours at 37 °C. The number of viable cells is determined using the Cell Proliferation Kit II. For cell-cycle analysis, MOLM-13 and RS4;11 cells are treated with KW-2449. After 24, 48, and 72 hours of incubation at 37 °C, DNA contents are analyzed. Cell cycle distribution of K562, TCC-Y, and TCC/Ysr is analyzed 24 hours after treatment with KW-2449 or imatinib. (Only for Reference)
Cell lines: MOLM-13 and RS4;11 cells
Animal Research
Animal Model: CBySmn.CB17-Prkdsscid/J (BALB/C) mice are injected with BV173/E255K/Luc cl4 cells.
Synonyms Takeda 779
Purity 98.00%
Molecular Weight 531.13
Formula C33H39ClN2O2
CAS No. 229005-80-5


-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 25 mg/mL (47.07 mM)

Ethanol: 62 mg/mL (186.5 mM)

Water: <1 mg/mL

H2O: 16.66 mg/mL (31.37 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

0.5% methylcellulose: 30 mg/mL


References and Literature
1. Suzaki Y , Hamada K , Nomi T , et al. A small-molecule compound targeting CCR5 and CXCR3 prevents airway hyperresponsiveness and inflammation[J]. European Respiratory Journal, 2008, 31(4):783-789. 2. Takama Y , Miyagawa S , Yamamoto A , et al. Effects of a calcineurin inhibitor, FK506, and a CCR5/CXCR3 antagonist, TAK-779, in a rat small intestinal transplantation model[J]. Transplant Immunology, 2011, 25(1):0-55.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Clinical Compound Library Stem cell Differentiation Compound Library Autophagy Compound Library Epigenetics Compound Library Tyrosine kinase inhibitor library Kinase Inhibitor Library Anti-cancer Clinical Compound Library Angiogenesis related Compound Library JAK STAT Compound Library Cell cycle related Compound Library

Related Products

Related compounds with same targets
PF-06700841 (P-Tosylate) NVP-AEW541 Nilotinib (monohydrochloride monohydrate) Brevilin A Kurarinone SP-146 Sorafenib Sorafenib tosylate

Dose Conversion

Safe and effective drug dosing is necessary, regardless of its purpose of administration. Learn More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Average weight of animals
Dosing volume per animal
Number of animals
Step Two: Enter the in vivo formulation
% Tween 80
% ddH2O
Calculate Reset


Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator

Molarity Calculator allows you to calculate the

  • mass of a compound required to prepare a solution of known volume and concentration
  • volume of solution required to dissolve a compound of known mass to a desired concentration
  • concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box


Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box


Calculate the volume of solvent required to reconstitute your vial

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.


Tech Support

Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.