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UAMC-3203

Catalog No. T5343 CAS 2271358-64-4

Synonyms: UAMC 3203, UAMC3203

UAMC-3203 is a potent and selective Ferroptosis inhibitor (IC50: 12 nM).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

UAMC-3203, CAS 2271358-64-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 31.00
5 mg	In stock	$ 70.00
10 mg	In stock	$ 112.00
25 mg	In stock	$ 188.00
50 mg	In stock	$ 283.00
100 mg	In stock	$ 422.00

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Purity: 100%

Purity: 98.81%

Purity: 98.55%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	UAMC-3203 is a potent and selective Ferroptosis inhibitor (IC50: 12 nM).
Targets&IC50	Ferroptosis:12 nM ( in IMR32 Neuroblastoma cells)
In vitro	UAMC-3203 exhibits even lower IC50-values (10 nM in IMR32 Neuroblastoma cells.) combined with an excellent kinetic solubility (> 200 μM).
In vivo	In blood plasma of mice having received a daily injection of compound UAMC-3203 over a period of 4 weeks, Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), LDH, creatine kinase (CK), creatinine, urea and troponin T levels were all comparable to background values detected in control mice.
Cell Research	In order to determine IC50-values, human neuroblastoma cells (IMR-32) were seeded in a 96-well plate at a density of 25,000 cells/well. The next day, the cells were pretreated for 1h (in triplicates) with a 1/3 dilution series of ferrostatin-1 analogues ranging from 5μM to 0.68nM and Sytox Green (1.6 μM) and a 1/2 dilution series of ferrostatin-1 analogues ranging from 200 nM to 0.78nM and Sytox Green (1.6 μM) for erastin and ferrous ammonium sulphate respectively. After stimulating the cells with erastin (10 μM) or ferrous ammonium sulphate (600 μM), the plate was transferred to a temperature- and CO2-controled FLUOstar Omega fluorescence plate reader. Sytox Green intensity was measured after 13h using an excitation filter of 485 nm and an emission filter of 520 nm. In each setup, Triton-X100 (0.05%) was used to induce lyses of the cells in 6 wells/plate, and was used as 100% cell death reference. The percentage of the cell death was calculated by the formula ((AVG[erastin] - AVG[background]) /(AVG[Triton-X100] - AVG[background])) × 100. Cell death percentage was plotted in GraphPad Prism 6, and IC50-values were calculated using a sigmoidal dose-response (variable slope) curve.
Animal Research	Vehicle solution (2% DMSO) or compound was administered daily at a concentration of 2 mM (in 0.9% NaCl containing 2% DMSO; 200 μL / 20 g body weight) by intraperitoneal injection. Body temperature and weight were monitored daily. On day 28 the mice were anesthetized with isoflurane and blood was sampled. Hereafter, mice were sacrificed by cervical dislocation. ALT, AST and LDH levels in plasma were measured. CK, creatinine, urea and troponin T were determined.

Synonyms	UAMC 3203, UAMC3203
Molecular Weight	471.66
Formula	C25H37N5O2S
CAS No.	2271358-64-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 199.3 mM

References and Literature

1. Devisscher L, et al. Discovery of Novel, Drug-Like Ferroptosis Inhibitors with in Vivo Efficacy. J Med Chem. 2018 Nov 21;61(22):10126-10140.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library NO PAINS Compound Library Apoptosis Compound Library Cell Cycle Compound Library Anti-COVID-19 Compound Library Bioactive Compound Library Bioactive Compounds Library Max Ferroptosis Compound Library

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Keywords

UAMC-3203 2271358-64-4 Apoptosis Ferroptosis UAMC 3203 inhibit Inhibitor UAMC3203 inhibitor

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