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XMD8-92

Catalog No. T1843 CAS 1234480-50-2

Purity 98.19% Datasheet

XMD8-92 is an effective and specific BMK1/ERK5 inhibitor (Kd: 80 nM).

XMD8-92, CAS 1234480-50-2

Pack Size	Availability	Price/USD
5 mg	In stock	60.00
10 mg	In stock	95.00
25 mg	In stock	220.00
50 mg	In stock	367.00
100 mg	In stock	590.00
1 mL * 10 mM (in DMSO)	In stock	60.00

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Purity 98.19%

HNMR HPLC COA Datasheet MSDS

Purity 98.00%

HNMR COA Datasheet MSDS

Biological Description

Chemical Properties

Storage & Solubility Information

Description	XMD8-92 is an effective and specific BMK1/ERK5 inhibitor (Kd: 80 nM).
Targets&IC50	BMI-1 :ic50 80nM(Kd),
Kinase Assay	KiNativ profiling of XMD8-92 is carried out with both an ATP and ADP acylphosphate-desthiobiotin with the following modifications. HeLa cell lysates (5 mg/mL total protein) are incubated in the presence of XMD8-92 at 50 μM, 10 μM, 2 μM, 0.8 μM, and 0 μM for 15 minutes prior to addition of the ATP or ADP acylphosphate probe (5 μM final probe concentration). All reactions are performed in duplicate. Probe reactions proceeded for 10 minutes and the reaction stopped by the addition of urea and processed for MS analysis. Samples are analyzed by LC-MS/MS on a linear ion trap mass spectrometer using a time segmented “target list” designed to collect MS/MS spectra from all kinase peptide-probe conjugates that can be detected in HeLa cell lysates. This target list is generated and validated by prior exhaustive analysis of HeLa lysates. Up to four characteristic fragment ions for each kinase peptide-probe conjugate are used to extract signals for each kinase, and a comparison of inhibitor treated to control (untreated) lysates allow for precise determination of % inhibition at each point. A manuscript describing the details of this targeted mass spectrometry approach is in preparation[1].
Animal Research	Animal Model: Mice

Molecular Weight	474.55
Formula	C26H30N6O3
CAS No.	1234480-50-2

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 19 mg/mL (40 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature

1. Yang Q, et al. Cancer Cell. 2010, 18(3), 258-267. 2. Dong H, et al. Pharm Biol. 2013. doi:10.3109/13880209. 3. Sureban SM, et al. Cancer Lett. 2014 , 351(1), 151-161.

Related compound libraries

This product is contained In the following compound libraries：

Bioactive Compound Library

Related Products

Related compounds with same targets

PTC596 PTC-028 XMD8-92 PTC209 PRT 4165 PTC-209 HBr

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Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.