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Z-DEVD-FMK

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Purity:99.69%

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COA HPLC HNMR

Z-DEVD-FMK

Catalog No. T6005Cas No. 210344-95-9

Z-DEVD-FMK (Caspase-3 Inhibitor) is a selective, irreversible Caspase-3 inhibitor, and also exhibits effective inhibition activity on caspase-6, caspase-7, caspase-8, and caspase-10.

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Pack Size	Price	Availability
1 mg	$52	In Stock
5 mg	$146	In Stock
10 mg	$246	In Stock
25 mg	$417	In Stock
50 mg	$595	In Stock
1 mL x 10 mM (in DMSO)	$216	In Stock

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Product Introduction

Bioactivity

Description	Z-DEVD-FMK (Caspase-3 Inhibitor) is a selective, irreversible Caspase-3 inhibitor, and also exhibits effective inhibition activity on caspase-6, caspase-7, caspase-8, and caspase-10.
In vivo	Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery. [2]
Kinase Assay	Caspase activity assay : Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade
Cell Research	N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference)
Alias	Caspase-3 Inhibitor

Chemical Properties

Molecular Weight	668.66
Formula	C30H41FN4O12
Cas No.	210344-95-9

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 50 mg/mL (74.78 mM)

5% DMSO+95% Saline: 4.65 mg/mL (6.95 mM, precipitation)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

Solution Preparation Table

DMSO/5% DMSO+95% Saline

	1mg	5mg	10mg	50mg
1 mM	1.4955 mL	7.4776 mL	14.9553 mL	74.7764 mL
5 mM	0.2991 mL	1.4955 mL	2.9911 mL	14.9553 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.1496 mL	0.7478 mL	1.4955 mL	7.4776 mL
20 mM	0.0748 mL	0.3739 mL	0.7478 mL	3.7388 mL
50 mM	0.0299 mL	0.1496 mL	0.2991 mL	1.4955 mL
100 mM	0.0150 mL	0.0748 mL	0.1496 mL	0.7478 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc