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Z-DEVD-FMK

Catalog No. T6005 CAS 210344-95-9

Synonyms: Caspase-3 Inhibitor

Z-DEVD-FMK (Caspase-3 Inhibitor) is a selective, irreversible Caspase-3 inhibitor, and also exhibits effective inhibition activity on caspase-6, caspase-7, caspase-8, and caspase-10.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Z-DEVD-FMK, CAS 210344-95-9

Pack Size	Availability	Price/USD
1 mg	In stock	$ 52.00
5 mg	In stock	$ 146.00
10 mg	In stock	$ 246.00
25 mg	In stock	$ 417.00
50 mg	In stock	$ 595.00
1 mL * 10 mM (in DMSO)	In stock	$ 216.00

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Purity: 99.69%

Purity: 96.19%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Z-DEVD-FMK (Caspase-3 Inhibitor) is a selective, irreversible Caspase-3 inhibitor, and also exhibits effective inhibition activity on caspase-6, caspase-7, caspase-8, and caspase-10.
Targets&IC50	Caspase-3:18 μM.
In vivo	Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery. [2]
Kinase Assay	Caspase activity assay : Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade
Cell Research	N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference)

Synonyms	Caspase-3 Inhibitor
Molecular Weight	668.66
Formula	C30H41FN4O12
CAS No.	210344-95-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 93 mg/mL (139.1 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Rickers A, et al. Eur J Immunol. 1998, (1), 296-304. 2. Yakovlev AG, et al. J Neurosci. 1997, 17(19), 7415-7424. 3. Wang J, et al. J Card Fail. 2000, 6(3), 243-249. 4. Meguro T, et al. Stroke. 2001, 32(2), 561-566. 5. Kanthasamy AG, et al. Free Radic Biol Med. 2006, 41(10), 1578-1589. 6. Zhang Y, Fan B Y, Pang Y L, et al. Neuroprotective effect of deferoxamine on erastininduced ferroptosis in primary cortical neurons[J]. Neural Regeneration Research. 2020, 15(8): 1539. 7. Zhang Y, Fan B Y, Pang Y L, et al. Neuroprotective effect of deferoxamine on erastin-induced ferroptosis in primary cortical neurons[J]. Neural regeneration research. 2020, 15(8): 1539. 8. Wang X Y, Wu K H, Pang H L, et al. Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera)[J]. International journal of molecular sciences. 2019, 20(18): 4325. 9. Liu X, Song X, Luan D, et al. Real-time in situ Visualizing the Sequential Activation of Caspase Cascade Using a Multi-color Au-Se Bonding Fluorescent Nanoprobe[J]. Analytical chemistry. 2019 May 7;91(9):5994-6002. 10. Zhu Q, Ding L, Zi Z, et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis[J]. Cell reports. 2019 Mar 26;26(13):3657-3671.e5.

Citations

1. Tang Q, Li W, Zheng X, et al. MELK is an oncogenic kinase essential for metastasis, mitotic progression, and programmed death in lung carcinoma. Signal Transduction and Targeted Therapy. 2020, 5(1): 1-12. 2. Zou Y, Zheng S, Xie X, et al. N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer. Nature Communications. 2022, 13(1): 1-18 3. Zhao Q, Feng H, Yang Z, et al. The central role of a two‐way positive feedback pathway in molecular targeted therapies‐mediated pyroptosis in anaplastic thyroid cancer. Clinical and Translational Medicine. 2022, 12(2): e727 4. Zhao Q, Feng H, Yang Z, et al. The central role of a two‐way positive feedback pathway in molecular targeted therapies‐mediated pyroptosis in anaplastic thyroid cancer. Clinical and Translational Medicine. 2022, 12(2): e727 5. Zhu Q, Ding L, Zi Z, et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis. Cell Reports. 2019 Mar 26;26(13):3657-3671.e5 6. Liu X, Song X, Luan D, et al. Real-time in situ Visualizing the Sequential Activation of Caspase Cascade Using a Multi-color Au-Se Bonding Fluorescent Nanoprobe. Analytical Chemistry. 2019, 91(9): 5994-6002 7. Hussain M, Lu Y, Tariq M, et al. A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism. Iscience. 2022, 25(7): 104591. 8. Wang X Y, Wu K H, Pang H L, et al. Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera). International journal of molecular sciences. 2019, 20(18): 4325. 9. Xia H, Zhang Z, You F. Inhibiting ACSL1-Related Ferroptosis Restrains Murine Coronavirus Infection. Viruses. 2021, 13(12): 2383. 10. Zhang Y, Fan B Y, Pang Y L, et al. Neuroprotective effect of deferoxamine on erastininduced ferroptosis in primary cortical neurons. Neural Regeneration Research. 2020, 15(8): 1539

Related compound libraries

This product is contained In the following compound libraries：

Highly Selective Inhibitor Library Anti-Neurodegenerative Disease Compound Library Inhibitor Library Human Metabolite Library NO PAINS Compound Library Bioactive Compound Library Covalent Inhibitor Library Anti-COVID-19 Compound Library Pyroptosis Compound Library Protease Inhibitor Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Z-DEVD-FMK 210344-95-9 Apoptosis Proteases/Proteasome Caspase ZDEVDFMK Inhibitor Z DEVD FMK Caspase-3 Inhibitor inhibit inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.