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AS601245

🥰Excellent
Catalog No. T2343Cas No. 345987-15-7

AS601245 is an inhibitor of the c-Jun NH2-terminal kinase (JNK), with neuroprotective properties.

AS601245

AS601245

🥰Excellent
Purity: 99.02%
Catalog No. T2343Cas No. 345987-15-7
AS601245 is an inhibitor of the c-Jun NH2-terminal kinase (JNK), with neuroprotective properties.
Pack SizePriceAvailabilityQuantity
1 mg$41In Stock
5 mg$97In Stock
10 mg$172In Stock
25 mg$288In Stock
50 mg$428In Stock
100 mg$637In Stock
1 mL x 10 mM (in DMSO)$107In Stock
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Purity:99.02%
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Product Introduction

Bioactivity
Description
AS601245 is an inhibitor of the c-Jun NH2-terminal kinase (JNK), with neuroprotective properties.
Targets&IC50
JNK3 (human):70 nM, JNK1 (human):150nM, JNK2 (human):220nM
Kinase Assay
FLT3 phosphorylation: Leukemia cells are washed in phosphate-buffered saline (PBS), then lysed by resuspending the cells in lysis buffer (20 mM Tris pH 7.4, 100 mM NaCl, 1% Igepal, 1 mM EDTA, 2 mM NaVO4, plus Complete protease inhibitor KW-2449 for 30 minutes while rocking. The extract is clarified by centrifugation at 1.6 × 104?g and the supernatant is assayed for protein (Bio-Rad). A 50-μg aliquot is removed as a whole-cell lysate for analysis of STAT5, and the remainder is used for immunoprecipitation with anti-FLT3. Anti-FLT3 antibody is added to the extract for overnight incubation, then protein A sepharose is added for 2 additional hours. Separate sodium dodecyl sulfate–polyacrylamide electrophoresis (SDS-PAGE) gels for whole-cell lysate and immunoprecipates are run in parallel. After transfer to Immobilon membranes, immunoblotting is performed with antiphosphotyrosine antibody (4 g10) to detect phosphorylated FLT3 or, for the whole-cell lysate gels, with a rat monoclonal antibody against phosphorylated STAT5 (residue Y694) then stripped and reprobed with anti-FLT3 antibody to measure total FLT3. Proteins are visualized using chemiluminescence, exposed on Kodak BioMax XAR film, developed, and scanned using a Bio-Rad GS800 densitometer. The concentration of KW-2449 for which the phosphorylation of FLT3 or STAT5 is inhibited to 50% of its baseline (IC50) is determined using linear regression analysis of the dose response curves. For direct analysis of FLT3 and STAT5 in circulating blasts, peripheral blood is collected in heparinized tubes and promptly chilled on ice. Samples are centrifuged for 10 minutes at 900 g, at 4 °C. The plasma is removed and stored frozen at ?80 °C. The buffy coat is carefully transferred to ice-cold PBS, layered onto chilled Ficoll-Hypaque, and centrifuged for 5 minutes at 600 g, at 4 °C. All subsequent steps are carried out at 4 °C. Mononuclear cells are collected and washed rapidly once in red blood cell lysis buffer (0.155 M NH4Cl, 0.01 M KHCO3, 0.1 mM EDTA), then washed once in PBS. Cells are then lysed as described for FLT3 and STAT5 analysis.
Chemical Properties
Molecular Weight372.45
FormulaC20H16N6S
Cas No.345987-15-7
SmilesN#CC(c1nc2ccccc2s1)c1ccnc(NCCc2cccnc2)n1
Relative Density.1.366 g/cm3 (Predicted)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 50 mg/mL (134.25 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.6849 mL13.4246 mL26.8492 mL134.2462 mL
5 mM0.5370 mL2.6849 mL5.3698 mL26.8492 mL
10 mM0.2685 mL1.3425 mL2.6849 mL13.4246 mL
20 mM0.1342 mL0.6712 mL1.3425 mL6.7123 mL
50 mM0.0537 mL0.2685 mL0.5370 mL2.6849 mL
100 mM0.0268 mL0.1342 mL0.2685 mL1.3425 mL

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