Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

CDDO-Im

🥰Excellent
Catalog No. TQ0120Cas No. 443104-02-7
Alias TP-235, RTA-403, CDDO-Imidazolide

CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).

CDDO-Im

CDDO-Im

🥰Excellent
Purity: 100%
Catalog No. TQ0120Alias TP-235, RTA-403, CDDO-ImidazolideCas No. 443104-02-7
CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).
Pack SizePriceAvailabilityQuantity
1 mg$31In Stock
5 mg$72In Stock
10 mg$113In Stock
25 mg$222In Stock
50 mg$355In Stock
100 mg$538In Stock
500 mg$1,150In Stock
1 mL x 10 mM (in DMSO)$86In Stock
Bulk & Custom
Add to Cart
Questions
View More

Related Compound Libraries of "CDDO-Im"

Select Batch
Purity:100%
Contact us for more batch information
Resource Download
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).
Targets&IC50
PPARγ:344 nM (Ki), PPARα:232 nM (Ki)
In vitro
CDDO-Im effectively suppresses the proliferation of human leukemia and breast cancer cell lines (IC50 approximately 10-30 nM). In U937 leukemia cells, it induces monocytic differentiation, evidenced by increased CD11b and CD36 expression [1]. CDDO-Im treatment causes G2/M-phase cell cycle arrest and apoptosis in triple-negative breast cancer cell lines SUM159 and MDA-MB-231, significantly inhibiting CD24−/EpCAM+ cells in SUM159 tumorspheres. It also decreases sphere-forming efficiency and size in both primary and secondary cultures [2]. Moreover, CDDO-Im elevates Nrf2 protein levels, which increases the expression of antioxidant and detoxification genes regulated by Nrf2 [3].
In vivo
CDDO-Im exhibits strong inhibition of de novo inducible nitric oxide synthase (iNOS) expression in primary mouse macrophages. Additionally, it effectively suppresses the proliferation of B16 murine melanoma and L1210 murine leukemia cells in vivo. Administering 10 nM (5.4 μg) of CDDO-Im significantly impedes the induction of iNOS by IFN-γ, while a dose as low as 1 nmol (0.54 μg) of CDDO-Im shows partial effectiveness [1].
Cell Research
CDDO-Im is dissolved in DMSO. SUM159 and MDA-MB-231 cells are seeded into each well of 96-well plates (1,000 cell/well) and treated the next day with vehicle control or CDDO-Im (1, 10, 50, 100 and 200 nM) for given incubation time. The absorbance is measured with a spectrophotometer to determine the cell proliferation rate [2].
Animal Research
Mice are injected i.p. with thioglycollate, and the resulting resident peritoneal macrophages are activated 3 days later with an i.p. injection of IFN-γ. CDDO and CDDO-Im are injected i.p. 30 min after IFN-γ. Macrophages are harvested 10 h later, cultured for 12 h, and then assayed for expression of iNOS protein and production of nitric oxide (NO) [1].
AliasTP-235, RTA-403, CDDO-Imidazolide
Chemical Properties
Molecular Weight541.72
FormulaC34H43N3O3
Cas No.443104-02-7
Smiles[H][C@@]12CC(C)(C)CC[C@@]1(CC[C@]1(C)[C@]2([H])C(=O)C=C2[C@@]1(C)CC[C@@]1([H])C(C)(C)C(=O)C(=C[C@]21C)C#N)C(=O)n1ccnc1
Relative Density.1.23 g/cm3 (Predicted)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 40 mg/mL (73.84 mM), Sonication is recommended.
H2O: Insoluble
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.8460 mL9.2299 mL18.4597 mL92.2986 mL
5 mM0.3692 mL1.8460 mL3.6919 mL18.4597 mL
10 mM0.1846 mL0.9230 mL1.8460 mL9.2299 mL
20 mM0.0923 mL0.4615 mL0.9230 mL4.6149 mL
50 mM0.0369 mL0.1846 mL0.3692 mL1.8460 mL

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
%Tween 80
%ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy CDDO-Im | purchase CDDO-Im | CDDO-Im cost | order CDDO-Im | CDDO-Im chemical structure | CDDO-Im in vivo | CDDO-Im in vitro | CDDO-Im formula | CDDO-Im molecular weight