Cycloheximide (Naramycin A) is a natural product that reversibly inhibits translation elongation, is an antimitotic compound, and is an inhibitor of DNA and protein synthesis.

Protein biosynthesis:532.5 nM.

Cycloheximide is used for inhibiting protein synthesis. Cycloheximide has shown that it can block the extension of the nuclear translation. Cycloheximide binds to the nucleus and inhibit EEF2 -mediated transliteration. [1]

Before training with 200 μA electric shock, mice were injected at 30, 60, or 120 mg/kg Cycloheximide. Cycloheximide has a significant impact on the incubation period of memory testing. In the control group, the delay of the test is significantly higher than the delay in training. The lowest dose of Cycloheximide test, 30 mg/kg, resulting in the incubation period of the test significantly higher than the control group. Accepting two higher doses of Cycloheximide, any kind of mice in the test are equivalent to the control group, the higher doses nor damage the memory under these conditions, resulting in the inverted U -shaped dose reaction curve. [2]