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Autophinib

Catalog No. T4219 CAS 1644443-47-9

Autophinib is a potent autophagy inhibitor with a novel chemotype with IC50 values of 90 and 40 nM for autophagy in starvation induced autophagy assay and rapamycin induced autophagy assay.

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Autophinib, CAS 1644443-47-9

Pack Size	Availability	Price/USD
1 mg	In stock	$ 30.00
2 mg	In stock	$ 43.00
5 mg	In stock	$ 68.00
10 mg	In stock	$ 101.00
25 mg	In stock	$ 180.00
50 mg	In stock	$ 288.00
100 mg	In stock	$ 450.00
500 mg	In stock	$ 987.00
1 mL * 10 mM (in DMSO)	In stock	$ 81.00

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Purity: 99.41%

Purity: 98.83%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Autophinib is a potent autophagy inhibitor with a novel chemotype with IC50 values of 90 and 40 nM for autophagy in starvation induced autophagy assay and rapamycin induced autophagy assay.
Targets&IC50	Autophagy:40 nM, Autophagy:90 nM
In vitro	Autophinib targets VPS34 but not other lipid kinases, mTOR and TBK1. Autophinib appears to be ATP-competitive, targets VPS34 in cell lysate and induces cellular phenotypes consistent with VPS34 inhibition. Autophinib inhibits the accumulation of autophagosomes under fed as well as starved conditions, but does not affect the number of autolysosomes. Thus it is not a late stage autophagy inhibitor but an inhibitor of autophagosome formation.
Cell Research	Cell lines: HeLa cellsConcentrations: 30 μMIncubation Time: 18 hMethod:

Molecular Weight	346.73
Formula	C14H11ClN6O3
CAS No.	1644443-47-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 64 mg/mL (184.6 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Robke L, et al. Phenotypic Identification of a Novel Autophagy Inhibitor Chemotype Targeting Lipid Kinase VPS34. Angew Chem Int Ed Engl. 2017 Jul 3;56(28):8153-8157.

Related compound libraries

This product is contained In the following compound libraries：

Kinase Inhibitor Library Inhibitor Library Anti-Ovarian Cancer Compound Library Anti-Colorectal Cancer Compound Library Neuronal Differentiation Compound Library Oxidation-Reduction Compound Library Anti-Pancreatic Cancer Compound Library Anti-Breast Cancer Compound Library Cancer Cell Differentiation Compound Library Neural Regeneration Compound Library

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(2S,3R,4S)-4-Hydroxyisoleucine Deguelin SRX3177 PI-3065 NU 7026 PI3Kα/mTOR-IN-1 PI3K/Akt/mTOR-IN-2 MOMIPP

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Autophinib 1644443-47-9 Autophagy PI3K/Akt/mTOR signaling PI3K inhibit Phosphoinositide 3-kinase Inhibitor inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.