-20℃ 3 years powder
-80℃ 2 years in solvent
Danusertib is a small-molecule 3-aminopyrazole derivative with potential antineoplastic activity. Danusertib binds to and inhibits the Aurora kinases, which may result in cell growth arrest and apoptosis in tumor cells in which Aurora kinases are overexpressed.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
2 mg | In stock | 79.00 | |
5 mg | In stock | 121.00 | |
10 mg | In stock | 219.00 | |
25 mg | In stock | 411.00 | |
50 mg | In stock | 658.00 | |
1 mL * 10 mM (in DMSO) | In stock | 124.00 |
Description | Danusertib is a small-molecule 3-aminopyrazole derivative with potential antineoplastic activity. Danusertib binds to and inhibits the Aurora kinases, which may result in cell growth arrest and apoptosis in tumor cells in which Aurora kinases are overexpressed. |
Targets&IC50 | Abl :ic50 25nM, Aurora A :ic50 13nM, Aurora B :ic50 79nM, Aurora C :ic50 61nM, FGFR1 :ic50 47nM, RET :ic50 31nM, TrkA :ic50 31nM, |
Kinase Assay | Biochemical kinase Assays: The Km values for ATP and the specific substrate are initially determined, and each assay is then run at optimized ATP (2Km) and substrate (5Km) concentrations. This setting enabled direct comparison of IC50 values of Danusertib across the applied kinase selectivity screening panel for the evaluation of the selectivity profile. |
Cell Research |
For short-term expansion assays, 1 × 103 CD34+ cells are plated in triplicates in 96-well plates containing 100 μL of serum-free medium per well supplemented with human stem-cell factor (100 ng/mL), human Flt-3 Ligand (100 ng/mL), human thrombopoietin (50 ng/mL), human interleukin-3 and -6 (IL-3 and IL-6, respectively, both 20 ng/mL), and granulocyte colony-stimulating factor (20 ng/mL) along with Danusertib at the indicated concentrations. After 5 days, an additional 100 μL of cytokine and Danusertib containing medium are added. Cell numbers within each individual well are estimated on days 3, 6, and 9 or on days 3, 6, and 12 for healthy donor samples. (Only for Reference)
Cell lines: CD34+ cells
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Animal Research |
Animal Model: FeSCID mice
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Synonyms | PHA-739358 |
Purity | 98.44% |
Molecular Weight | 474.55 |
Formula | C26H30N6O3 |
CAS No. | 827318-97-8 |
-20℃ 3 years powder
-80℃ 2 years in solvent
DMSO: 88 mg/mL (185.4 mM)
Ethanol: <1 mg/mL
Water: <1 mg/mL
( < 1 mg/ml refers to the product slightly soluble or insoluble )
1% DMSO+30% polyethylene glycol+1% Tween 80: 30 mg/mL
Safe and effective drug dosing is necessary, regardless of its purpose of administration. Learn More
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Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.