Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ezetimibe (SCH 58235) is a Dietary Cholesterol Absorption Inhibitor. The physiologic effect of ezetimibe is by means of Decreased Cholesterol Absorption.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 mg | In stock | $ 41.00 | |
25 mg | In stock | $ 56.00 | |
50 mg | In stock | $ 70.00 | |
100 mg | In stock | $ 89.00 | |
200 mg | In stock | $ 127.00 | |
500 mg | In stock | $ 208.00 | |
1 g | In stock | $ 297.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 50.00 |
Description | Ezetimibe (SCH 58235) is a Dietary Cholesterol Absorption Inhibitor. The physiologic effect of ezetimibe is by means of Decreased Cholesterol Absorption. |
Kinase Assay | GST-p62 is prepared from Escherichia coli and 0.5 μg of the purified GST-p62 protein is used for in vitro AMPK phosphorylation assay. Phosphorylation of p62 protein by AMPK is determined by non-radioisotope method using γS-ATP. AMPK complex is immuno-purified from the HEK293 cells, to which either myc-AMPKα1 wild-type (WT) or myc-AMPKα1 kinase-dead mutant (KD, D157A) is transfected with Flag-AMPKβ1 and HA-AMPKγ1. AMPK complex is added into the reaction mixture containing 20 mM HEPES, pH7.4, 1 mM EGTA, 0.4 mM EDTA, 5 mM MgCl2, 0.05 mM DTT, 0.5 μg GST-p62, 0.2 mM AMP, and 1 mM ATPγS. Reaction is carried out at 37°C for 30 min, and then terminated by adding 20 mM EDTA. To detect γS-labeled p62 protein, the reaction product is alkylated with 2.5 mM PNBM for 2 h at room temperature and analyzed the products by western blotting using anti-thiophosphate antibody[1]. |
Cell Research | Ezetimibe is dissolved in DMSO and stored, and then diluted with appropriate medium before use[2]. Huh7 human hepatocytes are cultured in high glucose DMEM containing 10% FBS, 100 units/mL penicillin and 100 μg/mL streptomycin at 37°C in a 95% air/5% CO2 atmosphere. Hepatocytes are treated with or without Ezetimibe (10 μM, 1 h) and incubated with palmitic acid (PA, 0.5 mM, 24 h)[2]. |
Synonyms | SCH 58235 |
Molecular Weight | 409.43 |
Formula | C24H21F2NO3 |
CAS No. | 163222-33-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 75 mg/mL (183.2 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 75 mg/mL (183.2 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Ezetimibe 163222-33-1 Autophagy Immunology/Inflammation Membrane transporter/Ion channel Others NPC1L1 Nrf2 SCH58235 inhibit Keap1-Nrf2 Inhibitor SCH 58235 SCH-58235 inhibitor