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IWP-2

Catalog No. T2702 CAS 686770-61-6

Synonyms: Casein Kinase, IWP2, Wnt, Inhibitor, IWP-2, IWP 2, Porcupine, inhibit

IWP-2, an inhibitor of Wnt processing and secretion (IC50=27 nM) in a cell-free assay, selectively blocks Porcn-mediated Wnt palmitoylation.

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IWP-2, CAS 686770-61-6

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Purity: 98%

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Description	IWP-2, an inhibitor of Wnt processing and secretion (IC50=27 nM) in a cell-free assay, selectively blocks Porcn-mediated Wnt palmitoylation.
Targets&IC50	Wnt:27 nM
In vivo	To evaluate the efficacy of IWP-2 in vivo, 200 μL each of IWP-2-liposome or free liposome i separately injected into C57BL/6 mice intraperitoneally about 2 h before injection of a similar volume of either blue-dye-filled latex beads or E. coli DH5α. IWP-2 causes significant reduction in the uptake of blue beads as well as E. coli as assessed by CFUs in peritoneal lavage cells within 2 h. In addition, the levels of TNF-α and IL-6 in the lavage fluid of the corresponding mice are reduced by 2-4-fold compared with control values. Interestingly, IWP-2 even induces a considerable increase in secretion of the anti-inflammatory cytokine IL-10[3]. Pretreatment with IWP-2 significantly (P<0.05) abolished SP-induced increase of Wnt3a, p-GSK3β, and β-catenin expressions[4].
Cell Research	IWP-2 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. The human RCC cell lines 786O and Caki-2 (5×103) are seeded into 96-well plates. Cell viability is estimated by MST assay after Caki-2 acells are incubated with ncreasing concentrations of LEF together with 20 μM IWP-2 for 48 h.After treatment, 10 μL MTS is added into each well for 2 h incubation. The absorbance is measured using a model ELX800 Micro Plate Reader at 490 nm. For colony formation assay, Caki-2 cells are trypsinized to single cell suspensions and seeded into fresh 6-well plates at 1000 cells/well. Then cells are incubated with LEF at depicted concentrations for 7 days. Colonies are fixed with absolute methanol for 15 min and then stained with 0.1% crystal violet for 20 min. After washing with PBS three times, the colonies with a diameter over 2 mm are visualized by a digital camera[2].

Synonyms	Casein Kinase, IWP2, Wnt, Inhibitor, IWP-2, IWP 2, Porcupine, inhibit
Molecular Weight	466.59
Formula	C22H18N4O2S3
CAS No.	686770-61-6

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 2mg/ml（4.29mM）

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature

1. Chen B, et al. Nat Chem Biol, 2009, 5(2), 100-107. 2. Maiti G, et al. The Wingless homolog Wnt5a stimulates phagocytosis but not bacterial killing. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16600-5. 3. Liu JD, et al. Wnt/Glycogen Synthase Kinase 3β/β-catenin Signaling Activation Mediated Sevoflurane Preconditioning-induced Cardioprotection. Chin Med J (Engl). 2015 Sep 5;128(17):2346-53.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Cancer Cell Differentiation Compound Library Anti-Obesity Compound Library Inhibitor Library DNA Damage & Repair Compound Library Wnt/Hedgehog/Notch Compound Library Neural Regeneration Compound Library Anti-Breast Cancer Compound Library Kinase Inhibitor Library Stem Cell Differentiation Compound Library

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