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Ionomycin calcium

Catalog No. T11665 CAS 56092-82-1

Synonyms: Ca2+ channels, PKC, ionophore, Calcium Channel, Ionomycin, SQ 23377, Ionomycin calcium, Antibiotic, Bacterial, SQ23377 calcium, Ca channels, Apoptosis, exosome, inhibit, SQ23377, Inhibitor, cleavage, Protein kinase C, calcium, SQ-23377

lonomycin calcium is a potent, selective calcium ionophore and an antibiotic produced by Streptomyces conglobatus. also induces the activation of protein kinase C (PKC). Ionomycin calcium is highly specific for divalent cations (Ca>Mg>Sr=Ba). Ionomycin promotes apoptosis.

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Ionomycin calcium, CAS 56092-82-1

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Purity: 98%

Biological Description

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Description

In vitro

Addition of 2 μM Ionomycin to LCLC 103H cells causes an instantaneous increase in intracellular Ca2+ concentration from 50 to 180 nM. DNA and protein analysis in Ionomycin-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typical of caspase-mediated apoptosis. Necrosis could be detected in ~1-5% of the Ionomycin treated cells as supported by simultaneously positive fluorescein labeling and propidium iodide uptake. Caspase activation in whole cells was followed by monitoring the increase in activity against Ac-DEVD-amc following Ionomycin treatment.

Synonyms	Ca2+ channels, PKC, ionophore, Calcium Channel, Ionomycin, SQ 23377, Ionomycin calcium, Antibiotic, Bacterial, SQ23377 calcium, Ca channels, Apoptosis, exosome, inhibit, SQ23377, Inhibitor, cleavage, Protein kinase C, calcium, SQ-23377
Molecular Weight	749.08
Formula	C41H72CaO9
CAS No.	56092-82-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 9 mg/mL (12.05 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature

1. Liu C,et al. Characterization of ionomycin as a calcium ionophore. J Biol Chem. 1978 Sep 10;253(17):5892-4. 1. Chen X C, Tang G X, Luo W H, et al. Monitoring and Modulating mtDNA G-Quadruplex Dynamics Reveal Its Close Relationship to Cell Glycolysis. Journal of the American Chemical Society. 2021 2. Chatila T, et al. Mechanisms of T cell activation by the calcium ionophore ionomycin. J Immunol. 1989 Aug 15;143(4):1283-9. 3. Gil-Parrado S, et al. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J Biol Chem. 2002 Jul 26;277(30):27217-26.

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