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MHY1485

Catalog No. T1908 CAS 326914-06-1

MHY1485 is a mTOR activator. It inhibits the autophagic process by inhibition of fusion between autophagosomes and lysosomes, leading to the accumulation of LC3II protein and enlarged autophagosomes.

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MHY1485, CAS 326914-06-1

Pack Size	Availability	Price/USD
5 mg	In stock	$ 43.00
10 mg	In stock	$ 61.00
25 mg	In stock	$ 113.00
50 mg	In stock	$ 196.00
100 mg	In stock	$ 363.00
200 mg	In stock	$ 535.00
1 mL * 10 mM (in DMSO)	In stock	$ 48.00

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Purity: 100%

Purity: 98%

Purity: 97.74%

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Description	MHY1485 is a mTOR activator. It inhibits the autophagic process by inhibition of fusion between autophagosomes and lysosomes, leading to the accumulation of LC3II protein and enlarged autophagosomes.
In vivo	MHY1485 elevates the phosphorylation levels of mTOR and its downstream targets, S6K1 and rpS6 proteins, without affecting the total content of mTOR, S6K1, and rpS6. Short-term treatment with MHY1485 before xenotransplantation of ovaries can promote the growth of secondary hair follicles. Additionally, post-treatment transplantation with MHY1485 facilitates the generation of mature oocytes and the production of healthy offspring. MHY1485 induces the accumulation of LC3II and the expansion of autophagosomes in a dose- and time-dependent manner.
Kinase Assay	Ovaries from mice at day10 of age are treated with 10 μM MHY1485 for 3h and proteins are extracted using M-PER Mammalian Protein Extraction Reagent containing a protease inhibitor cocktail. Protein concentrations in supernatants are determined by the bicinchoninic acid method. Equal amounts of protein lysates are loaded on 4-12% NuPAGE Bis-Tris gels in MOPS buffer and transferred to 0.45 μM pore nitrocellulose membranes[2].
Cell Research	MHY1485 is dissolved in DMSO and then diluted with appropriate media[3]. MC3T3-E1 cells are maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 mg/mL streptomycin at 37°C in a humidified atmosphere of 5% CO2. Having reached 70% confluence, the culture medium is switched to commercial osteogenic differentiation medium. MC3T3-E1 cells are cultured in the osteogenic differentiation medium for 14 days, following by culture in DMEM supplemented with varying concentrations of liraglutide (catalog no. HY-P0014; MedChem Express) for a further 14 days. MC3T3-E1 cells treated with 4 nM liraglutide are cultured in the presence or absence of Compound C or MHY1485. MC3T3-E1 cells maintained in DMEM for 28 days in the absence of any treatment are used as the negative control (NC); cells cultured in commercial osteogenic differentiation medium for 14 days and in DMEM without liraglutide for an additional 14 days are used as the positive control (PC)[3].

Molecular Weight	387.39
Formula	C17H21N7O4
CAS No.	326914-06-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 9 mg/mL(23.2 mM)

References and Literature

1. Choi YJ, et al. PLoS One. 2012, 7(8), e43418. 2. Cheng Y, et al. PLoS One.2015, 10(2), e0117769.

Citations

1. Hu J, Ling Z, Li W, et al.Glutamine promotes the proliferation of epithelial cells via mTOR/S6 pathway in oral lichen planus.Journal of Oral Pathology & Medicine.2022 2. Yang Z, Guo D, Zhao J, et al.Aggf1 Specifies Hemangioblasts at the Top of Regulatory Hierarchy via Npas4l and mTOR-S6K-Emp2-ERK Signaling.Arteriosclerosis, Thrombosis, and Vascular Biology.2023

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Kinase Inhibitor Library PI3K-AKT-mTOR Compound Library Anti-Cancer Metabolism Compound Library Target-Focused Phenotypic Screening Library Anti-Colorectal Cancer Compound Library Anti-Prostate Cancer Compound Library Glycolysis Compound Library Neuronal Differentiation Compound Library Anti-Obesity Compound Library

Related Products

Related compounds with same targets

9,9'-Di-O-(E)-feruloylsecoisolariciresinol mTOR inhibitor-14 CC-115 WYE-687 CCT128930 hydrochloride PI3Kα/mTOR-IN-1 MKC-1 Jolkinolide B

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Keywords

MHY1485 326914-06-1 Autophagy PI3K/Akt/mTOR signaling mTOR cell MC3T3-E1 FSH MHY 1485 Inhibitor cell-permeable Mammalian target of Rapamycin inhibit MHY-1485 inhibitor

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