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ML324

Catalog No. T6593 CAS 1222800-79-4

ML324(IC50=920 nM) is a specific inhibitor of jumonji histone demethylase (JMJD2).

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ML324, CAS 1222800-79-4

Pack Size	Availability	Price/USD
5 mg	In stock	$ 47.00
10 mg	In stock	$ 72.00
25 mg	In stock	$ 130.00
50 mg	In stock	$ 232.00
100 mg	In stock	$ 417.00
1 mL * 10 mM (in DMSO)	In stock	$ 76.00

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Purity: 98.22%

Purity: 97.49%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	ML324(IC50=920 nM) is a specific inhibitor of jumonji histone demethylase (JMJD2).
Targets&IC50	JMJD2:920 nM
In vitro	ML324 exhibits good Caco-2 cell permeability, and possesses excellent microsomal stability in the presence of both mouse and rat liver microsomes. ML324 demonstrates potent anti-viral activity against both herpes simplex virus (HSV) and human cytomegalovirus (hCMV) infection by inhibiting viral IE gene expression. [1]
In vivo	In a mouse ganglia explant model of latently infected mice, ML324 suppresses the formation of HSV plaques, and blocks HSV-1 reactivation. [1]
Kinase Assay	The fluorescence resonance energy transfer (FRET)-based LANCE Ultra KinaSelect Ser/Thr kit is used to determine IC50 values for various CDK inhibitors. Briefly, a specific ULight MBP peptide substrate (50 nM final concentration) is phosphorylated by a CDK-cyclin pair in buffer (50 mM HEPES-KOH pH 7.5, 10 mM MgCl2, 1 mM EGTA, 2 mM dithiothreitol) containing ATP at the concentration of the Km values of the individual kinases for 1 h at room temperature. Subsequently, phosphorylation is detected by addition of specific Eu-labelled anti-phospho-antibodies (2 nM), which upon binding to the phosphopeptide give rise to a FRET signal. FRET signals are then recorded[1].

Molecular Weight	349.43
Formula	C21H23N3O2
CAS No.	1222800-79-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 3 mg/mL (8.58 mM), Heating is recommended.

DMSO: 40 mg/mL (114.5 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Rai G, et al. Probe Reports from the NIH Molecular Libraries Program, 2010-2012.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Epigenetics Compound Library Anti-Aging Compound Library Histone Modification Compound Library Anti-Viral Compound Library Chromatin Modification Compound Library Bioactive Compound Library NO PAINS Compound Library Stem Cell Differentiation Compound Library Anti-Infection Compound Library

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LSD1-IN-5 LSD1-IN-25 JIB-04 JQKD82 GSK-J1 lithium salt GSK-LSD1 dihydrochloride Kgp-IN-1 Ryuvidine

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

ML324 1222800-79-4 Chromatin/Epigenetic Microbiology/Virology Others HSV Histone Demethylase Jumonji periodontal Cytomegalovirus inhibit simplex CMV Inhibitor Herpes simplex virus human disease H3K9 herpes ML 324 virus domain immunity periodontitis inflammation ML-324 containing proteins inhibitor

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