Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 39.00 | |
10 mg | In stock | $ 51.00 | |
25 mg | In stock | $ 92.00 | |
50 mg | In stock | $ 155.00 | |
100 mg | In stock | $ 239.00 | |
200 mg | In stock | $ 355.00 | |
500 mg | In stock | $ 589.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 43.00 |
Description | Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy. |
Targets&IC50 | Dnm1 GTPase:1 μM-10 μM |
In vitro |
METHODS: Human breast cancer cells MDA-MB-231 were treated with Mdivi-1 (5-40 μM) for 8 h. Mitotic spindle abnormalities were analyzed using the Immunostaining method. RESULTS: Mdivi-1 dose-dependently increased the percentage of mitotic cells with spindle abnormalities. [1] METHODS: Human lung cancer cells H460, A549 and human colorectal cancer cells HCT116 were treated with Mdivi-1 (20-50 μM) for 24 h. Cell death was detected by Flow Cytometry. RESULTS: Mdivi-1 significantly inhibited the death of the three tumor cells. [2] |
In vivo |
METHODS: To assay in vivo activity, Mdivi-1 (50 mg/kg) was injected intraperitoneally into C57BL/6 mice, and transient retinal ischemia was subsequently induced by acute IOP elevation. RESULTS: Mdivi-1 treatment blocked apoptotic cell death in the ischemic retina and significantly increased retinal ganglion cell survival two weeks after ischemia. [3] METHODS: To investigate the effects on experimental autoimmune encephalomyelitis (EAE), Mdivi-1 (25 mg/kg) was administered intraperitoneally to C57BL/6 mice induced with EAE once daily for twenty-five days. RESULTS: Mdivi-1 effectively suppressed the severity of EAE by reducing demyelination and cellular infiltration in the central nervous system.Mdivi-1 has therapeutic potential in EAE by modulating the balance between Th1/Th17 and regulatory T cells. [4] |
Kinase Assay | All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity. |
Cell Research | Mdivi-1 is dissolved in DMSO. YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system. |
Synonyms | Mitochondrial division inhibitor 1 |
Molecular Weight | 353.22 |
Formula | C15H10Cl2N2O2S |
CAS No. | 338967-87-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 35.3 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
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Mdivi-1 338967-87-6 Apoptosis Autophagy Cytoskeletal Signaling Mitophagy Dynamin Mitochondrial division inhibitor 1 Mitochondrial Autophagy Mitochondrial division inhibitor1 Inhibitor inhibit Mdivi 1 Mdivi1 Mitochondrial division inhibitor-1 inhibitor