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Mdivi-1

Catalog No. T1907   CAS 338967-87-6
Synonyms: Mitochondrial division inhibitor 1

Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.

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Mdivi-1 Chemical Structure
Mdivi-1, CAS 338967-87-6
Pack Size Availability Price/USD Quantity
5 mg In stock $ 39.00
10 mg In stock $ 51.00
25 mg In stock $ 92.00
50 mg In stock $ 155.00
100 mg In stock $ 239.00
200 mg In stock $ 355.00
500 mg In stock $ 589.00
1 mL * 10 mM (in DMSO) In stock $ 43.00
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Purity: 98.79%
Purity: 98.64%
Purity: 98.32%
Purity: 98.24%
Purity: 98.22%
Purity: 98.072%
Purity: 98%
Purity: 96.57%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.
Targets&IC50 Dnm1 GTPase:1 μM-10 μM
In vitro METHODS: Human breast cancer cells MDA-MB-231 were treated with Mdivi-1 (5-40 μM) for 8 h. Mitotic spindle abnormalities were analyzed using the Immunostaining method.
RESULTS: Mdivi-1 dose-dependently increased the percentage of mitotic cells with spindle abnormalities. [1]
METHODS: Human lung cancer cells H460, A549 and human colorectal cancer cells HCT116 were treated with Mdivi-1 (20-50 μM) for 24 h. Cell death was detected by Flow Cytometry.
RESULTS: Mdivi-1 significantly inhibited the death of the three tumor cells. [2]
In vivo METHODS: To assay in vivo activity, Mdivi-1 (50 mg/kg) was injected intraperitoneally into C57BL/6 mice, and transient retinal ischemia was subsequently induced by acute IOP elevation.
RESULTS: Mdivi-1 treatment blocked apoptotic cell death in the ischemic retina and significantly increased retinal ganglion cell survival two weeks after ischemia. [3]
METHODS: To investigate the effects on experimental autoimmune encephalomyelitis (EAE), Mdivi-1 (25 mg/kg) was administered intraperitoneally to C57BL/6 mice induced with EAE once daily for twenty-five days.
RESULTS: Mdivi-1 effectively suppressed the severity of EAE by reducing demyelination and cellular infiltration in the central nervous system.Mdivi-1 has therapeutic potential in EAE by modulating the balance between Th1/Th17 and regulatory T cells. [4]
Kinase Assay All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity.
Cell Research Mdivi-1 is dissolved in DMSO. YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system.
Synonyms Mitochondrial division inhibitor 1
Molecular Weight 353.22
Formula C15H10Cl2N2O2S
CAS No. 338967-87-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 35.3 mg/mL (100 mM)

TargetMolReferences and Literature

1. Fang CT, et al. Mdivi-1 induces spindle abnormalities and augments taxol cytotoxicity in MDA-MB-231 cells. Cell Death Discov. 2021 May 20;7(1):118. 2. Dai W, et al. Mitochondrial division inhibitor (mdivi-1) decreases oxidative metabolism in cancer. Br J Cancer. 2020 Apr;122(9):1288-1297. 3. Park SW, et al. A selective inhibitor of drp1, mdivi-1, increases retinal ganglion cell survival in acute ischemic mouse retina. Invest Ophthalmol Vis Sci. 2011 Apr 27;52(5):2837-43. 4. Li YH, et al. Mdivi-1, a mitochondrial fission inhibitor, modulates T helper cells and suppresses the development of experimental autoimmune encephalomyelitis. J Neuroinflammation. 2019 Jul 19;16(1):149.

TargetMolCitations

1. Li Q, Chu Y, Li S, et al. The oncoprotein MUC1 facilitates breast cancer progression by promoting Pink1-dependent mitophagy via ATAD3A destabilization. Cell Death & Disease. 2022, 13(10): 1-16. 2. Guo Q, Zhang Y C, Wang W, et al. Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic stroke via inducing a unique post-translational hypusination modification. Pharmacological Research. 2022: 106046. 3. Guo Q, Zhang Y C, Wang W, et al. Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic stroke via inducing a unique post-translational hypusination modification. Pharmacological Research. 2022: 106046. 4. Li C, Pan Y, Tan Y, et al. PINK1-Dependent Mitophagy Reduced Endothelial Hyperpermeability and Cell Migration Capacity Under Simulated Microgravity. Frontiers in cell and developmental biology. 2022, 10. 5. Shi Y, Liu Q, Chen W, et al. Protection of Taohong Siwu Decoction on PC12 cells injured by oxygen glucose deprivation/reperfusion via mitophagy-NLRP3 inflammasome pathway in vitro. Journal of Ethnopharmacology. 2022: 115784. 6. Wang J, Su Q, Wu Q, et al. Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells. Archives of Pharmacal Research. 2021: 1-12. 7. Su Q, Wang J, Liu F, et al. Blocking Parkin/PINK1-mediated mitophagy sensitizes hepatocellular carcinoma cells to sanguinarine-induced mitochondrial apoptosis. Toxicology in Vitro. 2020: 104840 8. Su Q, Wu Q, Chen K, et al. Induction of Estrogen Receptor β-mediated Autophagy Sensitizes Breast Cancer Cells to TAD1822-7, a Novel Biphenyl Urea Taspine Derivative. Molecular Biology Reports. 2021 9. Ma L, Chen C, Hai S, et al.Inhibition of Mitochondrial Fission Alleviates Zearalenone-Induced Mitochondria-Associated Endoplasmic Reticulum Membrane Dysfunction in Piglet Sertoli Cells.Toxins.2023, 15(4): 253. 10. Wang H, Ye J, Peng Y, et al.CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction.Autophagy.2023 (just-accepted).
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Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compounds Library Max Inhibitor Library Bioactive Compound Library Apoptosis Compound Library Cuproptosis Compound Library Immunology/Inflammation Compound Library NO PAINS Compound Library Mitochondria-Targeted Compound Library Covalent Inhibitor Library Autophagy Compound Library

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Keywords

Mdivi-1 338967-87-6 Apoptosis Autophagy Cytoskeletal Signaling Mitophagy Dynamin Mitochondrial division inhibitor 1 Mitochondrial Autophagy Mitochondrial division inhibitor1 Inhibitor inhibit Mdivi 1 Mdivi1 Mitochondrial division inhibitor-1 inhibitor

 

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