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PRT062607 hydrochloride

Catalog No. T2696 CAS 1370261-97-4

Synonyms: PRT062607 (P505-15, BIIB057) HCl, P505-15 Hydrochloride

PRT062607 hydrochloride (P505-15 Hydrochloride) is a selective inhibitor of Syk (IC50: 1 nM). It displays at least 80-fold selectivity for Syk over other kinases.

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PRT062607 hydrochloride Chemical Structure

PRT062607 hydrochloride, CAS 1370261-97-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 55.00
2 mg	In stock	$ 78.00
5 mg	In stock	$ 129.00
10 mg	In stock	$ 197.00
25 mg	In stock	$ 344.00
50 mg	In stock	$ 527.00
200 mg	In stock	$ 997.00
1 mL * 10 mM (in DMSO)	In stock	$ 142.00

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Purity: 99.93%

Purity: 97.7%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	PRT062607 hydrochloride (P505-15 Hydrochloride) is a selective inhibitor of Syk (IC50: 1 nM). It displays at least 80-fold selectivity for Syk over other kinases.
Targets&IC50	FGR:81 nM (cell free), MLK1:88 nM (cell free), Syk:1 nM (cell free)
In vitro	PRT062607 (P505-15) is a highly specific and potent inhibitor of purified Syk (IC50 1-2 nM). In human whole blood, P505-15 potently inhibited B cell antigen receptor-mediated B cell signaling and activation (IC50 0.27 and 0.28 μM, respectively) and Fcε receptor 1-mediated basophil degranulation (IC50 0.15 μM). Similar levels of ex vivo inhibition were measured after dosing in mice (Syk signaling IC50 0.32 μM) [1]. P505-15 abrogated the pro-survival effect of anti-IgM and induced CLL cell apoptosis. Treatment with P505-15 (2 mM/mL) decreased CLL cell viability to 62.9 ± 5.1% after 48 hours. Treatment with lower concentrations of PRT318 and P505-15 also reduced the viability of CLL cells [2].
In vivo	Demonstrating a rapid onset of action, 0.5 h after oral administration of 30 mg/kg P505-15, Syk activity was reduced by 80% and nearly completely suppressed for the next 5 h relative to vehicle control-treated mice. Syk activity remained more than 60% suppressed for the first 8 h postdosing, returning to levels of vehicle control treatment by 24 h. At the lower dose of 15 mg/kg, more than 50% suppression of Syk activity was observed between 2 and 6 h after oral administration of the compound [1].
Kinase Assay	Potency of Syk inhibition was determined by using a fluorescence resonance energy transfer (FRET) assay. The extent of substrate phosphorylation by Syk was measured in the presence of various P505-15 concentrations. Syk activity was determined by a fluorescent antibody specific for phosphorylated tyrosine by using the increase of FRET. Twelve concentrations were tested for dose response. Specificity and potency of kinase inhibition was determined by evaluation of P505-15 in the Millipore KinaseProfiler panel of 270 independent purified kinase assays. For profiling, P505-15 was tested in duplicate at two concentrations at a fixed concentration of ATP. Subsequently, IC50 determinations using the radioactive assays were carried out at an ATP concentration optimized for each individual kinase. All radioactive ATP incorporation enzyme assays were performed at Millipore UK [1].
Cell Research	NLC co-cultures were established by suspending PBMC from patients with CLL in complete RPMI medium with 10% fetal bovine serum and penicillin-streptomycin-glutamine to a concentration of 107 cells/mL (total 2 mL). Cells were incubated for 14 days in 24-well plates as previously described. To evaluate whether the Syk inhibitors PRT318 and P505-15 could overcome the protective effect of NLC, CLL cells were cultured under standardized conditions on NLC or in suspension, in the presence or absence of PRT318 and P505-15. At the indicated time points, CLL cells were collected and assayed for cell viability as previously described [2].
Animal Research	All animal studies were performed in strict accordance with the Institutional Animal Care and Use Committee ethical guidelines. Female BALB/c mice received a single oral dose of 15 or 30 mg/kg P505-15 and were anesthetized with a subcutaneous ketamine cocktail, and blood was harvested via cardiac puncture at 0.5, 1, 2, 3, 4, 5, 6, 8, and 24 h postdose, (n = 3/time point; n = 8 vehicle controls). Blood was dispensed into three heparin-containing tubes, one for determination of drug concentration and the remaining two for ex vivo stimulation with isotype control or anti-mouse IgD antibody for 10 min. Blood was processed for intracellular phospho-flow cytometry to evaluate BCR signaling as described earlier; mouse B cells were detected by using CD45R-B220 PerCP-conjugated antibody. Plasma samples were analyzed for P505-15 concentration by using a liquid chromatography tandem mass spectrometer. The analytical range was 2 to 5000 ng/ml [1].

Synonyms	PRT062607 (P505-15, BIIB057) HCl, P505-15 Hydrochloride
Molecular Weight	429.91
Formula	C19H23N9O·HCl
CAS No.	1370261-97-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: 79 mg/mL (183.8 mM)

DMSO: 80 mg/mL (186.1 mM)

References and Literature

1. Coffey G, et al. Specific inhibition of spleen tyrosine kinase suppresses leukocyte immune function and inflammation in animal models of rheumatoid arthritis. J Pharmacol Exp Ther. 2012 Feb;340(2):350-9. 2. Hoellenriegel J, et al. Selective, novel spleen tyrosine kinase (Syk) inhibitors suppress chronic lymphocytic leukemia B-cell activation and migration. Leukemia. 2012 Jul;26(7):1576-83.

Related compound libraries

This product is contained In the following compound libraries：

Tyrosine Kinase Inhibitor Library Inhibitor Library Drug Repurposing Compound Library Highly Selective Inhibitor Library Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library Clinical Compound Library Anti-Ovarian Cancer Compound Library Anti-Colorectal Cancer Compound Library Anti-Pancreatic Cancer Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Keywords

PRT062607 hydrochloride 1370261-97-4 Angiogenesis MAPK Tyrosine Kinase/Adaptors MLK Src Syk inhibit PRT 062607 PRT062607 PRT062607 (P505-15, BIIB057) HCl PRT-062607 Inhibitor Spleen tyrosine kinase PRT-062607 hydrochloride PRT-062607 Hydrochloride PRT062607 Hydrochloride P505-15 PRT 062607 Hydrochloride P505-15 Hydrochloride inhibitor

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