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Puromycin aminonucleoside

Catalog No. T16684 CAS 58-60-6

Synonyms: NSC 3056

Puromycin aminonucleoside (NSC-3056) increases podocyte permeability by modulating ZO-1 in an oxidative stress-dependent manner.

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Puromycin aminonucleoside Chemical Structure

Puromycin aminonucleoside, CAS 58-60-6

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1 mL * 10 mM (in DMSO)	In stock	$ 43.00

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Purity: 99.97%

Purity: 99.88%

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Storage & Solubility Information

Description	Puromycin aminonucleoside (NSC-3056) increases podocyte permeability by modulating ZO-1 in an oxidative stress-dependent manner.
In vitro	Puromycin aminonucleoside -induced podocyte apoptosis is p53 dependent. Puromycin aminonucleoside causes podocyte apoptosis in a time-dependent manner. The IC50 values for PMAT-expressing and vector-transfected cells are 48.9 and 122.1 μM, respectively, suggesting expression of PMAT-enhanced cell sensitivity to Puromycin aminonucleoside. Puromycin aminonucleoside uptake in PMAT-expressing cells is fourfold higher at pH 6.6 than that at pH 7.4. Puromycin aminonucleoside (30 μg/mL) markedly enhances p53 protein levels in podocytes. Puromycin aminonucleoside (250 μM) is toxic to both PMAT-expressing and vector-transfected cells [2][4].
In vivo	Rats administered Puromycin aminonucleoside (100 mg/kg, s.c.) exhibited reduced weight gain and elevated serum creatinine levels compared to controls. The podocyte count per glomerulus in control rats stood at 95.5±17.6, dropping to 90.7 by Day 4 in rats with Puromycin aminonucleoside (8 mg/100 g, i.v.)-induced nephrosis. Furthermore, nephrin levels per glomerulus in controls were 1.02±0.11 fmol, which decreased significantly in nephrosis rats to 0.46±0.06 fmol and 0.35±0.04 fmol on Days 4 and 7, respectively. This reduction in nephrin per podocyte was closely linked to proteinuria development in rats affected by Puromycin aminonucleoside nephrosis [5][6].

Synonyms	NSC 3056
Molecular Weight	294.31
Formula	C12H18N6O3
CAS No.	58-60-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 33.33 mg/mL (113.25 mM), Sonification is recommended.

DMSO: 31 mg/mL(105.33 mM)

References and Literature

1. Lacalle RA, et al. Cloning of the complete biosynthetic gene cluster for an aminonucleoside antibiotic, puromycin, and its regulated expression in heterologous hosts. EMBO J. 1992 Feb;11(2):785-92. 2. Wada T, et al. Prevents podocyte apoptosis induced by puromycin aminonucleoside: role of p53 and Bcl-2-related family proteins. J Am Soc Nephrol. 2005 Sep;16(9):2615-25. 3. Gong W, et al. Estrogen-related receptor-α mediates puromycin aminonucleoside-induced mesangial cell apoptosis and inflammatory injury. Am J Physiol Renal Physiol. 2019 May 1;316(5):F906-F913. 4. Xia L, et al. Podocyte-specific expression of organic cation transporter PMAT: implication in puromycin aminonucleosidenephrotoxicity. Am J Physiol Renal Physiol. 2009 Jun;296(6):F1307-13. 5. Kawakami H, et al. Dynamics of absolute amount of nephrin in a single podocyte in puromycin aminonucleoside nephrosis rats calculated by quantitative glomerular proteomics approach with selected reaction monitoring mode. Nephrol Dial Transplant. 2012 Apr; 6. Nosaka K, et al. An adenosine deaminase inhibitor prevents puromycin aminonucleoside nephrotoxicity. Free Radic Biol Med 1997 ;22 (4): 597-605.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Apoptosis Compound Library Anti-Infection Compound Library HIF-1 Signaling Pathway Compound Library Bioactive Compound Library Human Metabolite Library Metabolism Compound Library Antibiotics Library Ubiquitination Compound Library Covalent Inhibitor Library

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Keywords

Puromycin aminonucleoside 58-60-6 Apoptosis Metabolism Microbiology/Virology Proteases/Proteasome Ubiquitination Proteasome Aminopeptidase Antibacterial Antibiotic NSC-3056 Bacterial Inhibitor inhibit NSC3056 DPP Dipeptidyl Peptidase NSC 3056 inhibitor

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