Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RG108 (N-Phthalyl-L-tryptophan) is an DNA methyltransferase inhibitor(IC50=115 nM).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 39.00 | |
10 mg | In stock | $ 63.00 | |
25 mg | In stock | $ 119.00 | |
50 mg | In stock | $ 216.00 | |
100 mg | In stock | $ 302.00 | |
200 mg | In stock | $ 423.00 | |
500 mg | In stock | $ 687.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 51.00 |
Description | RG108 (N-Phthalyl-L-tryptophan) is an DNA methyltransferase inhibitor(IC50=115 nM). |
Targets&IC50 | DNA methyltransferase:115 nM |
In vivo | In iPS cells derived from SM, RG108 significantly reduces DNA activity. In tumor cells, RG108 inhibits gene demethylation and reactivation without affecting the methylation of telomeric gene sequences. |
Kinase Assay | In vitro methylation assay: The substrate DNA for the in vitro methylation assay is a 798 bp fragment (?423/+375 relative to the initiation codon) from the promoter region of the human p16Ink4a gene. The methylation reaction contains 350 to 400 ng substrate DNA and 4 units of M.SssI methylase (0.5 μM) in a final volume of 50 μL. Inhibitors are added to final concentrations of 10, 100, 200, and 500 μM, respectively. Reactions are done at 37 °C for 2 hours. After completion, the reaction is inactivated at 65 °C for 15 minutes and the DNA is purified using PCR Purification kit. Three hundred nanograms of purified DNA is digested for 3 hours at 60 °C with 30 units of BstUI and analyzed on 2% Tris-borate EDTA agarose gels. |
Cell Research | For the determination of cellular growth and viability, cells are stained with trypan blue and counted using a standard counting grid.(Only for Reference) |
Synonyms | N-Phthalyl-L-tryptophan |
Molecular Weight | 334.33 |
Formula | C19H14N2O4 |
CAS No. | 48208-26-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 33.4 mg/mL (100 mM)
DMSO: 33.4 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
RG108 48208-26-0 Chromatin/Epigenetic DNA Methyltransferase inhibit Inhibitor DNMTs N-Phthalyl-L-tryptophan DNA MTases RG 108 RG-108 inhibitor