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Sal003

Catalog No. T2417Cas No. 1164470-53-4

Sal003, an effective cell-permeable analog, inhibitis the eIF2α phosphatase.

Sal003

Sal003

Purity: 99.77%
Catalog No. T2417Cas No. 1164470-53-4
Sal003, an effective cell-permeable analog, inhibitis the eIF2α phosphatase.
Pack SizePriceAvailabilityQuantity
1 mg$32In Stock
2 mg$45In Stock
5 mg$67In Stock
10 mg$105In Stock
25 mg$192In Stock
50 mg$285In Stock
100 mg$423In Stock
1 mL x 10 mM (in DMSO)$68In Stock
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Purity:99.77%
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Product Introduction

Bioactivity
Description
Sal003, an effective cell-permeable analog, inhibitis the eIF2α phosphatase.
In vitro
In mouse embryonic fibroblasts, Sal003 induced depolymerization of polysaccharides by increasing the phosphorylation of eIF2a. Sal003 increased eIF2a phosphorylation by blocking eIF2a phosphodiesterase activity, and Sal003 enhanced the apoptotic signaling pathway induced by cytotoxicity by induced phosphorylation of eIF2α.
In vivo
In mouse embryonic fibroblasts, Sal003 induced depolymerization of polysaccharides by increasing the phosphorylation of eIF2a. Sal003 increased eIF2a phosphorylation by blocking eIF2a phosphodiesterase activity, and Sal003 enhanced the apoptotic signaling pathway induced by cytotoxicity by induced phosphorylation of eIF2α.
Kinase Assay
Catalytic assay: MEK5 protein isolated from the baculovirus expression system is used to measure kinase activity utilizing PKLight ATP Detection Reagent. The assay is performed using 15 nM GST-MEK5 and 0.75 μM ATP in assay buffer consisting of 25 mM Hepes, pH 7.5, 10 mM MgCl2, 50 mM KCl, 0.2% BSA, 0.01% CHAPS, 100 μM Na3VO4, 0.5 mM DTT and 1% DMSO in the presence of varying concentrations of BIX02189. The kinase reaction mixture is incubated for 90 minutes at room temperature followed by addition of 10 μL of ATP detection reagent for 15 minutes. The relative light unit (RLU) signal is measured and the RLU signals are converted to percent of control (POC) values for the determination of IC50 value.
Chemical Properties
Molecular Weight463.21
FormulaC18H15Cl4N3OS
Cas No.1164470-53-4
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 46.3 mg/mL (100 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.1588 mL10.7942 mL21.5885 mL107.9424 mL
5 mM0.4318 mL2.1588 mL4.3177 mL21.5885 mL
10 mM0.2159 mL1.0794 mL2.1588 mL10.7942 mL
20 mM0.1079 mL0.5397 mL1.0794 mL5.3971 mL
50 mM0.0432 mL0.2159 mL0.4318 mL2.1588 mL
100 mM0.0216 mL0.1079 mL0.2159 mL1.0794 mL

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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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