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Torin 1

Catalog No. T6045   CAS 1222998-36-8

Torin 1 is an effective inhibitor of mTORC1/2 with (IC50: 2 nM/10 nM); has 1000-fold selectivity for mTOR than PI3K.

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Torin 1 Chemical Structure
Torin 1, CAS 1222998-36-8
Pack Size Availability Price/USD Quantity
2 mg In stock $ 41.00
5 mg In stock $ 67.00
10 mg In stock $ 97.00
25 mg In stock $ 183.00
50 mg In stock $ 328.00
100 mg In stock $ 497.00
500 mg In stock $ 1,080.00
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Purity: 99.38%
Purity: 99%
Purity: 98.3%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Torin 1 is an effective inhibitor of mTORC1/2 with (IC50: 2 nM/10 nM); has 1000-fold selectivity for mTOR than PI3K.
Targets&IC50 mTORC1:2 nM, mTORC2:10 nM
Kinase Assay mTORC1 and mTORC2 in Vitro Kinase Assays: To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer. (Only for Reference)
Molecular Weight 607.62
Formula C35H28F3N5O2
CAS No. 1222998-36-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 2.5 mM

TargetMolReferences and Literature

1. Thoreen CC, et al, J Biol Chem, 2009, 284(12), 8023-8032. 2. Liu Q, et al, J Med Chem, 2010, 53(19), 7146-7155. 3. Li J, et al, Am J Physiol Cell Physiol, 2011, 301(1), C213-C226. 4. Dowling RJ, et al, Science, 2010, 328(5982), 1172-1176. 5. Mitra D, Vega‐Rubin‐de‐Celis S, Royla N, et al. Abrogating GPT2 in triple negative breast cancer inhibits tumor growth and promotes autophagy[J]. International Journal of Cancer. 6. Mitra D, Vega‐Rubin‐de‐Celis S, Royla N, et al. Abrogating GPT2 in triple‐negative breast cancer inhibits tumor growth and promotes autophagy[J]. International Journal of Cancer. 2021, 148(8): 1993-2009. 7. Lu X Y, Shi X J, Hu A, et al. Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis[J]. Nature. 2020, 588(7838): 479-484.

TargetMolCitations

1. Lu X Y, Shi X J, Hu A, et al. Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis. Nature. 2020, 588(7838): 479-484. 2. Sun C Y, Li Y Z, Cao D, et al. Rapamycin and trametinib: a rational combination for treatment of NSCLC. International Journal of Biological Sciences. 2021, 17(12): 3211-3223. 3. Mitra D, Vega‐Rubin‐de‐Celis S, Royla N, et al. Abrogating GPT2 in triple-negative breast cancer inhibits tumor growth and promotes autophagy. International Journal of Cancer. 2021 Apr 15;148(8):1993-2009. 4. Zheng J, Deng Y, Wei Z, et al.Lipid phosphatase SAC1 suppresses hepatitis B virus replication through promoting autophagic degradation of virions.Antiviral Research.2023: 105601. 5. Huang Z, Zhu S, Han Z, et al.Proteome-Wide Analysis Reveals TFEB Targets for Establishment of a Prognostic Signature to Predict Clinical Outcomes of Colorectal Cancer.Cancers.2023, 15(3): 744. 6. Li L, Fu S, Wang J, et al.SRT1720 inhibits bladder cancer cell progression by impairing autophagic flux.Biochemical Pharmacology.2024: 116111.

Related compound libraries

This product is contained In the following compound libraries:
Highly Selective Inhibitor Library Bioactive Compound Library PI3K-AKT-mTOR Compound Library Autophagy Compound Library HIF-1 Signaling Pathway Compound Library Anti-Lung Cancer Compound Library Metabolism Compound Library Cancer Cell Differentiation Compound Library Neural Regeneration Compound Library Anti-Obesity Compound Library

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Keywords

Torin 1 1222998-36-8 Autophagy DNA Damage/DNA Repair PI3K/Akt/mTOR signaling DNA-PK PI3K mTOR Mammalian target of Rapamycin Torin1 Inhibitor Torin-1 inhibit inhibitor

 

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