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YM-90709

Catalog No. T3133 CAS 163769-88-8

Synonyms: YM90709, YM 90709

YM-90709 is a new-type antagonist inhibiting the binding of interleukin-5 to the interleukin-5 receptor.

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YM-90709, CAS 163769-88-8

Pack Size	Availability	Price/USD
1 mg	In stock	$ 30.00
2 mg	In stock	$ 39.00
5 mg	In stock	$ 63.00
10 mg	In stock	$ 80.00
25 mg	In stock	$ 163.00
50 mg	In stock	$ 297.00
100 mg	In stock	$ 449.00
1 mL * 10 mM (in DMSO)	In stock	$ 68.00

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Purity: 99.67%

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Description	YM-90709 is a new-type antagonist inhibiting the binding of interleukin-5 to the interleukin-5 receptor.
In vitro	YM-90709 potently inhibits the binding of 100 pM [125I]-IL-5 to IL-5R on human peripheral eosinophils and eosinophilic HL-60 clone 15 cells with IC50 values of 1.0±0.40 and 0.57±0.21 μM, respectively. YM-90709 inhibits the 4 pM IL-5-induced effect in a concentration-dependent manner with an IC50 value of 0.45±0.024 μM. YM-90709 also inhibits the higher concentrations (12 and 40 pM) of IL-5-induced effects with IC50 values of 0.89±029 and 1.0±0.22 μM, respectively. [1] YM-90709 is a novel interleukin-5 receptor antagonist, YM-90709 inhibits antigen-induced eosinophil recruitment into the airway, the same as anti-IL-5 mAb does. YM-90709 inhibits the binding of IL-5 to IL-5R on human eosinophils, but did not inhibit the binding of GM-CSF to GM-CSFR. In addition, YM-90709 inhibits IL-5-induced, but not GM-CSF-induced, eosinophil survival as well as the tyrosine phosphorylation of Janus kinase 2. [2]
In vivo	YM-90709 suppresses antigen-induced airway inflammation in Brown Norway rats . YM-90709 is a novel IL-5R antagonist with those of anit-IL-5 mAb on the antigen-induced infiltration of eosinophils into the airways of BDF1 mice, a strain that is commonly used in the antibody estimation. [2] This is the first report on the examination of the effects of YM-90709 in vivo, as a novel IL-5R antagonist on the antigen-induced infiltration of eosinophils and other leukocytes into the BALF of Brown-Norway (BN) rats. [3]
Kinase Assay	Nuclease assay: Reactions with oligonucleotide nonhairpin substrates contains 25 mM MOPS (pH 7.0), 60 mM KCl, 0.2% Tween 20, 2 mM DTT, 1 mM or 5 MnCl2 (or 5 mM MgCl2, or 5 mM CaCl2), 0.1 pmol of DNA substrate, and 0.3 pmol of Mre11 (or an equivalent amount of Mre11 complexed with Rad50) in a volume of 10 μl, and are incubated at 37°C for 30 min. SDS, EDTA, and proteinase K are then added to final concentrations of 0.2%, 5 mM, and 0.1 mg/ml, respectively, and incubated for another 15 min. 4 μl of each reaction is mixed with 4 μl of formamide loading buffer, and then loaded onto a sequencing gel containing 10% acrylamide and 7 M urea. After the run, each gel is analyzed using a phosphorimaging system. Reactions containing hairpin substrates are identical to those with nonhairpin substrates except that 3 pmol of Mre11 is added to reactions as indicated, and the reactions are incubated at room temperature overnight. Nonhomologous end-joining reactions contains 25 mM MOPS (pH 7.0), 60 mM KCl, 0.2% Tween 20, 2 mM DTT, 4 mM MgCl2, 2 mM MnCl2, 0.5 mM ATP, 4 ng of plasmid DNA, 10% polyethylene glycol, 0.01 pmol of human DNA ligase I, and 0.06 pmol of Mre11 or 0.1 units of E. coli exonuclease III (GIBCO-BRL), in a volume of 10 μl. After incubation at 37°C for 25 min, Tween 20 is added to a final concentration of 0.5%, and a 2.5 μl aliquot is amplified by PCR using primers DAR5 and DAR147. PCR products are cloned using the TA cloning kit and sequenced using an automated ABI Capillary Genetic Analyzer.

Synonyms	YM90709, YM 90709
Molecular Weight	359.42
Formula	C22H21N3O2
CAS No.	163769-88-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 18 mg/mL(50 mM)

References and Literature

1. Morokata T, et al. Int Immunopharmacol. 2004 Jul;4(7):873-83. 2. Immunol Lett. 2005 Apr 15;98(1):161-5. Epub 2004 Nov 28. 3. Morokata T, et al. Int Immunopharmacol. 2002 Nov;2(12):1693-702.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compound Library Bioactive Compounds Library Max Cytokine Inhibitor Library Target-Focused Phenotypic Screening Library NO PAINS Compound Library HIF-1 Signaling Pathway Compound Library Immunology/Inflammation Compound Library Nonsteroidal Anti-Inflammatory Compound Library Pyroptosis Compound Library

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Keywords

YM-90709 163769-88-8 Immunology/Inflammation IL Receptor Interleukin YM90709 GM-CSF inhibit Inhibitor Interleukin Related IL-5 eosinophils IL-5R YM 90709 JAK2 inhibitor

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