keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective caspase 8 inhibitor, which has cell permeable.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 127.00 | |
2 mg | In stock | $ 191.00 | |
5 mg | In stock | $ 276.00 | |
10 mg | In stock | $ 443.00 | |
25 mg | In stock | $ 718.00 | |
50 mg | In stock | Inquiry | |
1 mL * 10 mM (in DMSO) | In stock | $ 429.00 |
Description | Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective caspase 8 inhibitor, which has cell permeable. |
In vitro | Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. [1] Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [2] Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. [3] |
In vivo | In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. [3] Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. [4] |
Kinase Assay | Proteasome assay: Exponentially growing cells on a 96-well clustered plate are treated with different concentrations of drugs or left untreated (control) for 6 hours. Proteasomes extracted with 0.5% NP40 buffer are mixed with equal amounts of samples in 100 μL total volume, and then incubated with 25 μmol/L of fluorogenic substrates (LRR- specific for trypsin-like activity, LLE-specific for caspase-like activity, and SUVY-specific for chymotrypsin-like activity) in black-bottom 96-well plates at 37°C. Fluorescence is monitored every 5 minutes at the wavelength of 360 nm (excitation) and 480 nm (emission). |
Synonyms | Z-IE(OMe)TD(OMe)-FMK, Caspase-8 Inhibitor |
Molecular Weight | 654.68 |
Formula | C30H43FN4O11 |
CAS No. | 210344-98-2 |
keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 84 mg/mL (128.3 mM)
You can also refer to dose conversion for different animals. More
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Z-IETD-FMK 210344-98-2 Apoptosis Proteases/Proteasome Caspase Z-IE(OMe)TD(OMe)-FMK Caspase-8 Inhibitor inhibit Inhibitor Z IETD FMK ZIETDFMK inhibitor