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Embelin

🥰Excellent
Catalog No. T6485Cas No. 550-24-3
Alias NSC 91874, Emberine, Embelic acid

Embelin (Embelic acid), isolated from the Japanese Ardisia herb, is an inhibitor of the X-linked inhibitor of apoptosis (IC50: 4.1 uM).

Embelin

Embelin

🥰Excellent
Purity: 99.88%
Catalog No. T6485Alias NSC 91874, Emberine, Embelic acidCas No. 550-24-3
Embelin (Embelic acid), isolated from the Japanese Ardisia herb, is an inhibitor of the X-linked inhibitor of apoptosis (IC50: 4.1 uM).
Pack SizePriceAvailabilityQuantity
10 mg$50In Stock
25 mg$85In Stock
50 mg$160In Stock
100 mg$314In Stock
1 mL x 10 mM (in DMSO)$50In Stock
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Purity:99.88%
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Product Introduction

Bioactivity
Description
Embelin (Embelic acid), isolated from the Japanese Ardisia herb, is an inhibitor of the X-linked inhibitor of apoptosis (IC50: 4.1 uM).
Targets&IC50
X linked:4.1 μM
In vitro
Embelin from the Japanese Ardisia herb is a small-molecular inhibitor that binds to the XIAP BIR3 domain with which Smac and caspsase-9 bind. Embelin inhibits cell growth of both PC-3 and LNCap cells in a dose-dependent manner, with IC50 values of 3.7 and 5.7 μM. While, the toxicity of Embelin in normal PrEC and in WI-38 cells is much lower with IC50 values of 20.1 μM and 19.3 μM. Treatment of PC-3 cells with 25 and 50 μM of embelin for 48 h, 30% and 75% of cells undergo apoptosis, representing approximately 3-and 9-fold increase as compared to untreated cells. [1] Embelin also potently suppresses the biosynthesis of eicosanoids by selective inhibition of 5-lipoxygenase (5-LO) and Microsomal prostaglandin E2 synthase-1 (mPGES-1) with IC50 = 0.06 and 0.2 mM, respectively. [2]
Kinase Assay
Fluorescence Polarization Competitive Binding Assay: Fluorescence polarization experiments are performed in Dynex 96-well, black, round-bottom plates. A 5 μL sample of Embelin dilutions in DMSO, and preincubated XIAP BIR3 protein (0.06 μM) and the N terminus of a Smac peptide (SM7F) (0.01 μM) in the assay buffer are added to 96-well plates to produce a final volume of 125 μL. For each assay, the bound peptide control containing XIAP BIR3 protein and SM7F (equivalent to 0% inhibition) and free peptide control containing only free SM7F (equivalent to 100% inhibition) were included. The plates were mixed and incubated at room temperature for 3 h to reach equilibrium.
Cell Research
Cell growth is determined by the MTT-based assay using Cell Proliferation Reagent WST-1 according to the manufacturer's instruction. Cells (5000 cells/well) are grown in medium with 10% FBS, and various concentrations of Embelin are added to the cells. Four to five days later, WST-1 is added to each well and incubated for 1-3 h at 37 °C. Absorbance is measured with a plate reader at 450 nm with correction at 650 nm.(Only for Reference)
AliasNSC 91874, Emberine, Embelic acid
Chemical Properties
Molecular Weight294.39
FormulaC17H26O4
Cas No.550-24-3
SmilesCCCCCCCCCCCC1=C(O)C(=O)C=C(O)C1=O
Relative Density.1.131 g/cm3
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Ethanol: 2.9 mg/mL (10 mM)), Heating is recommended.
DMSO: 29.4 mg/mL (100 mM)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM3.3969 mL16.9843 mL33.9685 mL169.8427 mL
5 mM0.6794 mL3.3969 mL6.7937 mL33.9685 mL
10 mM0.3397 mL1.6984 mL3.3969 mL16.9843 mL
DMSO
1mg5mg10mg50mg
20 mM0.1698 mL0.8492 mL1.6984 mL8.4921 mL
50 mM0.0679 mL0.3397 mL0.6794 mL3.3969 mL
100 mM0.0340 mL0.1698 mL0.3397 mL1.6984 mL

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