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Hesperetin

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Catalog No. T2565Cas No. 520-33-2

Hesperetin belongs to the flavanone class of flavonoids. Hesperetin, in the form of its glycoside hesperidin, is the predominant flavonoid in lemons and oranges.

Hesperetin

Hesperetin

🥰Excellent
Purity: 98.54%
Catalog No. T2565Cas No. 520-33-2
Hesperetin belongs to the flavanone class of flavonoids. Hesperetin, in the form of its glycoside hesperidin, is the predominant flavonoid in lemons and oranges.
Pack SizePriceAvailabilityQuantity
25 mg36 €In Stock
50 mg51 €In Stock
100 mg76 €In Stock
1 mL x 10 mM (in DMSO)47 €In Stock
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Purity:98.54%
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Product Introduction

Bioactivity
Description
Hesperetin belongs to the flavanone class of flavonoids. Hesperetin, in the form of its glycoside hesperidin, is the predominant flavonoid in lemons and oranges.
In vitro
Hesperetin has the retention of antioxidant potential in self nano-emulsifying drug delivery system[1]. Hesperetin and NGR display broad-spectrum inhibition against human UGTs. In addition, Hesperetin exhibits strong inhibitory effects on UGT1A1, 1A3 and 1A9 (both IC50 and Ki values lower than 10 μM) and moderately inhibits UGT1A4, UGT1A7, UGT1A8 (IC50 values 29.68-63.87 μM)[2]. Hesperetin interacts with different types of proteins involving hydrogen bonds, pi-pi effects, pi-cation bonding and pi-sigma interactions with varying binding energies. Hesperetin exhibits drug-like properties which indicate its potential as a therapeutic option for CHIKV infection[3]. Hesperetin dose-dependently reduces GCDCA-induced caspase-3 activity in cultured primary rat hepatocytes. Hesperetin also dose-dependently reduces CM-induced Nos2 (iNOS) expression in hepatocytes. Interestingly, hesperetin-induced expression of the antioxidant gene haem oxygenase 1 (HO-1) about fourfold compared with cytokine mixture alone[5].
In vivo
Pre-administration of Hesperetin (40 mg/kg b.w., oral) significantly reduces Cd-induced oxidative stress and mitochondrial dysfunction in rats’ brains, while restoring antioxidant activity and membrane-bound enzyme functions, and lessening apoptosis[4]. At a dose of 200 mg/kg, Hesperetin diminishes Con A-induced hepatocyte apoptosis and hepatic Nos2 (iNOS) expression in mice, alongside reducing the presence of apoptotic bodies, hydropic degeneration, nuclear fragments, autolysis, and hemorrhage. Moreover, Hesperetin co-treatment notably lowers the infiltration of leukocytes in the liver tissue of mice suffering from D-GalN/LPS-induced fulminant hepatitis, demonstrating its protective effect in a murine model[5].
Kinase Assay
First, 0.5 mL tissue homogenate is diluted with 1 mL water. Then, to this mixture, 2.5 mL ethanol and 1.5 mL chloroform (all reagents chilled) are added and shaken for 1 min at 4°C, then centrifuged. The enzyme activity in the supernatant is determined. The assay mixture contained 1.2 mL sodium pyrophosphate buffer (0.025 M, pH 8.3), 0.1 mL 186 mM phenazine methosulfate (PMS), 0.3 mL 30 mM Nitroblue tetrazolium (NBT), and 0.2 mL of nicotinamide adenine dinucleotide (NADH), and appropriately diluted enzyme preparation and water in a total volume of 3 mL. Reaction is initiated by the addition of NADH. After incubation at 30°C for 90 min, the reaction is stopped by the addition of 1 mL glacial acetic acid. The reaction mixture is stirred vigorously and shaken with 4 mL n-butanol. The intensity of the chromogen in the butanol layer is measured at 560 nm against a butanol blank. A system without enzyme served as control. One unit of enzyme activity is defined as 50% inhibition of NBT reduction in 1 min under the assay conditions.
Chemical Properties
Molecular Weight302.28
FormulaC16H14O6
Cas No.520-33-2
SmilesO=C1C=2C(O[C@@H](C1)C3=CC(O)=C(OC)C=C3)=CC(O)=CC2O
Relative Density.1.458g/cm3
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 45 mg/mL (148.87 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM3.3082 mL16.5410 mL33.0819 mL165.4096 mL
5 mM0.6616 mL3.3082 mL6.6164 mL33.0819 mL
10 mM0.3308 mL1.6541 mL3.3082 mL16.5410 mL
20 mM0.1654 mL0.8270 mL1.6541 mL8.2705 mL
50 mM0.0662 mL0.3308 mL0.6616 mL3.3082 mL
100 mM0.0331 mL0.1654 mL0.3308 mL1.6541 mL

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